›› 2002, Vol. 45 ›› Issue (3): 296-300.

• 研究论文 • 上一篇    下一篇

植物次生物质对烟青虫和棉铃虫食物利用及中肠解毒酶活性的影响

董钧锋,张继红,王琛柱   

  • 出版日期:2002-06-20 发布日期:2002-06-20

Effects of plant allelochemicals on nutritional utilization and detoxication enzyme activities in two Helicoverpa species

DONG Jun-Feng, ZHANG Ji-Hong, WANG Chen-Zhu*   

  • Online:2002-06-20 Published:2002-06-20

摘要: 用分别添加0-5%(干重比)棉酚、烟碱、番茄苷和辣椒素4种植物次生物质的人工饲料饲养烟青虫Helicoverpa assulta和棉铃虫H. armigera 5龄幼虫48 h,测定这些次生物质对烟青虫和棉铃虫的营养效应和中肠谷胱甘肽S-转移酶(GST)及羧酸酯酶(CarE)活性的影响。结果表明:在实验浓度下,棉酚可显著降低烟青虫的相对消化率,但对棉铃虫却有助食作用;番茄苷抑制烟青虫的取食和生长,对其近似消化率和食物利用率也有显著的抑制作用,但食物转化率有明显升高,对棉铃虫的各营养指标无显著影响;烟碱对烟青虫和棉铃虫的相对生长率均无影响;辣椒素使烟青虫的取食量有大幅度的提高,对棉铃虫的取食量无影响,但引起其相对消化率的提高。由此可见,棉铃虫对4种次生物质有普遍的适应性,而烟青虫只对寄主植物所含的烟碱和辣椒素有较好的适应性。烟青虫和棉铃虫幼虫中肠CarE活性不受4种次生物质的影响,烟碱和辣椒素对烟青虫GST有显著的诱导作用,番茄苷对烟青虫GST活性则有抑制作用,4种次生物质对棉铃虫GST均无显著影响。

关键词: 植物次生物质, 烟青虫, 棉铃虫, 营养指标, 谷胱甘肽-S-转移酶, 羧酸酯酶

Abstract: The effects of four plant allelochemicals,gossypol, nicotine, tomatine and capsicine, on nutritional utilization and detoxication enzymes activities in midgut were investigated with the 5th instar larvae of cotton bollworm, Helicoverpa armigera, and oriental tobacco budworm, H. assulta. The results showed that H. armigera exhibited wide adaptability to all the four allelochemicals, whereas H. assulta could only fit well to nicotine and capsicin, which were the allelochemicals from its host plants. Midgut CarE activity was unaffected by the allelochemicals in H. armigera and H. assulta. GST activity in the midgut of H. assulta could be strongly induced by nicotine and capsicine, but significantly inhibited by tomatine; none of the four allelochemicals could affect midgut GST activity in H. armigera.

Key words: plant allelochemicals, Helicoverpa assulta, H. armigera, nutritional indices, detoxication enzymes