›› 2002, Vol. 45 ›› Issue (3): 301-306.

• 研究论文 • 上一篇    下一篇

抑前胸腺肽在家蚕体内的活性作用

刘新1, 华跃进1*, 徐步进1, 田中良明2   

  • 出版日期:2002-06-20 发布日期:2002-06-20

In vivo activity of prothoracicostatic peptide in the silkworm, Bombyx mori

LIU Xin1, HUA Yue-Jin1*, XU Bu-Jin1,TANAKA Yoshia-ki2   

  • Online:2002-06-20 Published:2002-06-20

摘要: 家蚕Bombyx mori抑前胸腺肽是昆虫脑神经肽的一种,体外实验表明它能抑制处于活动时期的家蚕前胸腺合成蜕皮激素,因此抑前胸腺肽可能对昆虫的变态起着重要的作用。将抑前胸腺肽以不同的浓度分单一注射和加强注射导入家蚕体内,不同的时间间隔取样,利用蜕皮激素放射免疫分析方法,观察到了抑前胸腺肽在家蚕体内的活性作用以及引起家蚕体内血淋巴中蜕皮激素浓度的动态变化,首次证明了抑前胸腺肽在体内对家蚕前胸腺合成蜕皮激素有强烈的抑制作用。

关键词: 家蚕, 抑前胸腺肽, 蜕皮激素, 体内活性

Abstract: Prothoracicostatic peptide is one of insect brain neuropeptides, which was observed to inhibit the prothoracic glands of the silkworms, Bombyx mori, at active stage of ecdysone biosynthesis, in the experiments in vitro. In the present paper, prothoracicostatic peptide was injected into the silkworms in different dosages with different methods, single or strengthened injection, and hemolyms were taken thereafter in different intervals. By using 3H-ecdysteriod radioimmunoassay, a remarkable in vivo activity of prothoracicostatic peptide and developmental changes of ecdysteroid titer in the silkworm in vivo, caused by prothoracicostatic peptide treatment were gained. This is the first evidence that prothoracicostatic peptide significantly inhibits ecdysteroidogenesis in an insect in vivo.

Key words: Bombyx mori, prothoracicostatic peptide, ecdysteroid, in vivo activity