美国白蛾核型多角体病毒p35基因的克隆及序列分析

›› 2002, Vol. 45 ›› Issue (6): 711-716.

美国白蛾核型多角体病毒p35基因的克隆及序列分析

曹广力，薛仁宇，朱越雄，魏育红，贡成良

出版日期:2002-12-20 发布日期:2002-12-20

loning and nucleotide sequence analysis of the p35 gene of Hyphantria cunea nucleopolyhedrovirus

CAO Guang-Li, XUE Ren-Yu, ZHU Yue-Xiong, WEI Yu-Hong, GONG Cheng-Liang

Online:2002-12-20 Published:2002-12-20

摘要/Abstract

摘要： 对美国白蛾核型多角体病毒（HycuNPV，Hyphantria cunea nucleopolyhedrovirus）p35基因的序列分析表明：HycuNPV p35编码序列900?bp, 编码299氨基酸。同源性分析表明：HycuNPV p35与BomoNPV T3、AucaNPV、SpliNPV、LeseNPV、HearNPV在核苷酸水平上为99.9%、95.7%、93.6%、80.2%和87.2%，在氨基酸水平上为99.7%、90.3%、77%、64.9%和73.2%，显示了杆状病毒p35基因在进化上的保守性。BomoNPV T3中位的H122，在HycuNPV中被R取代。推测HycuNPV p35蛋白的功能及抑制细胞凋亡的能力与BomoNPV T3 p35蛋白的相似。

关键词: 美国白蛾核型多角体病毒, p35基因, 核苷酸序列

Abstract: Sequence analysis of the p35 gene of Hyphantria cunea nucleopolyhedrovirus (HycuNPV) showed that the HycuNPV p35 gene was 900 bp and encoded 299 amino acids. Compared with BomoNPV T3, AucaNPV, SpliNPV, LeseNPV and HearNPV, nucleotide and amino acid identity were 99.9%, 95.7%, 93.6%, 80.2%, 87.2% and 99.7%, 90.3%, 77%, 64.9%, 732% respectively, and showed evolutionary conservation. H¹²² of BomoNPV T3 p35 was replaced by R in HycuNPV p35. It is possible that the function and anti-apoptotic action of HycuNPV p35 is similar to that of BomoNPV T3 p35.

Key words: Hyphantria cunea nucleopolyhedrovirus, p35 gene, nucleotide sequence

曹广力，薛仁宇，朱越雄，魏育红，贡成良. 美国白蛾核型多角体病毒p35基因的克隆及序列分析[J]. , 2002, 45(6): 711-716.

CAO Guang-Li, XUE Ren-Yu, ZHU Yue-Xiong, WEI Yu-Hong, GONG Cheng-Liang. loning and nucleotide sequence analysis of the p35 gene of Hyphantria cunea nucleopolyhedrovirus[J]. , 2002, 45(6): 711-716.

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