›› 2002, Vol. 45 ›› Issue (6): 733-738.

• 研究论文 • 上一篇    下一篇

不同种群棉铃虫三龄幼虫酯酶活性频率分布与抗药性的关系

陈松, 杨亦桦, 吴益东   

  • 出版日期:2002-12-20 发布日期:2002-12-20

Frequency distributions of esterase activity and insecticide resistance in thirdinstar larvae in different strains of Helicoverpa armigera Hübner

CHEN Song, YANG Yi-Hua, WU Yi-Dong*   

  • Online:2002-12-20 Published:2002-12-20

摘要: 选用室内饲养的棉铃虫Helicoverpa armigera偃师和湖北2个敏感品系、对辛硫磷高抗的PCP20品系、对氰戊菊酯高抗的YG45品系及1999或2000年采自山东阳谷、河北邯郸和河南安阳的田间高抗种群,江苏徐州、湖北武汉的田间中等抗性水平种群和新疆沙湾的田间敏感种群,采用酶标板酶动力学法测定了各品系(种群)的3龄幼虫个体酯酶活性频率分布和平均酯酶活性。结果表明,偃师敏感品系、湖北敏感品系和新疆沙湾田间敏感种群的酯酶活性个体频率分布相似,三个品系(种群)的平均酯酶活性相近,分别为991、1 138、1 055 mOD·min-1·larva-1。室内选育的PCP20抗性品系、YG45抗性品系及山东阳谷、河北邯郸 、河南安阳田间高抗种群的高酯酶活性(活性在1 800 mOD·min-1·larva-1以上)个体频率明显高于三个敏感品系(种群),平均酯酶活性在1 510~2 482 mOD·min-1·larva-1之间。江苏徐州、湖北武汉的田间中抗水平种群高酯酶活性个体频率及平均酯酶活性都介于敏感和高抗品系(种群)之间,平均酯酶活性为1 258~1.404 mOD·min-1·larva-1。棉铃虫各品系(种群)平均酯酶活性与对拟除虫菊酯类杀虫剂抗性个体频率的相关性要比对有机磷类的高,相关系数分别为0.82和0.42。分析各品系(种群)高酯酶活性个体频率与棉铃虫对拟除虫菊酯类、有机磷类杀虫剂抗性个体频率的相关性,得到相似的结果。考虑到酯酶并不是棉铃虫对拟除虫菊酯抗性的主要机理,建议酯酶活性可作为棉铃虫抗药性生化检测的一个参考指标。本文还讨论了酯酶与棉铃虫对拟除虫菊酯类杀虫剂及有机磷类杀虫剂抗性的关系。

关键词: 棉铃虫, 酯酶, 抗药性, 生化检测

Abstract: General esterase activity to α-NA in two susceptible laboratory strains (Yanshi and Hubei), two lab-selected resistant strains (PCP20 and YG45) and nine field strains of cotton bollworm Helicoverpa armigera Hübner were measured by microtiter plate kinetic assay. Frequency distribution patterns of esterase activity in the Yanshi, Hubei and Shawan susceptible field strains were similar and their mean esterase activities were 991, 1 138 and 1 055 mOD·min-1·larva-1 respectively. Two labselected resistant strains (PCP20, YG45) and three field resistant strains (Yanggu, Handan and Anyang) had many more individuals of high esterase activity (more than 1 800 mOD·min-1·larva-1) than three susceptible strains and their mean esterase activities ranged from 1 510 to 2 482 mOD·min-1·larva-1. Another two field strains (Xuzhou and Wuhan) had moderate-high easterase individuals and their mean esterase activities were 1 208 and 1 404 mOD·min-1·larva-1. The correlation coefficient between mean esterase activity and resistance level was 0.82 to pyrethroids and 0.42 to organophosphates. The same results were obtained when the correlation between the frequency of individuals with high esterase activity and the resistance levels to pyrethriod and organophosphate was analyzed. The results suggested that general esterase activity has some relationship to pyrethroid resistance levels. Because esterase is not a major mechanism for pyrethroid resistance in H. armigera it can only be used as a reference index for the detection of biochemical resistance.

Key words: Helicoverpa armigera, esterase, insecticide resistance, biochemical detection