›› 2002, Vol. 45 ›› Issue (6): 748-752.

• 研究论文 • 上一篇    下一篇

新型非甾醇蜕皮激素类杀虫剂对棉铃虫幼虫蜕皮的影响

杜育哲1, 郭世宜1, 王秀玲1, 刘安西1, 汪清民2, 黄润秋   

  • 出版日期:2002-12-20 发布日期:2002-12-20

The effects of a new type of nonsteroidal ecdysteroid agonist on cuticle formation in larvae of the cotton bollworm(Helicoverpa armigera)

DU Yu-Zhe1, GUO Shi-Yi1, WANG Xiu-Ling1, LIU An-Xi1, WANG Qing-Min2, HUANG Run-Qiu2   

  • Online:2002-12-20 Published:2002-12-20

摘要: 用透射电镜技术研究了新型非甾醇蜕皮激素类杀虫剂W200013对棉铃虫4龄幼虫蜕皮的影响。结果表明W200013使棉铃虫产生早熟、致死的蜕皮。中毒试虫6 h表现出内表皮层沉积加快,皮细胞中粗面型内质网大量增加,糖原颗粒减少;12 h蜕皮间隙开始形成,细胞质凝集;24 h新、旧表皮同时存在,皮细胞空泡化严重;36 h新上表皮覆盖于仅沉积几层的新原表皮上,旧表皮仍然保持,皮细胞呈恶化、降解状态,宏观上虫体不表现出蜕皮行为而死亡。而且比较研究可见W200013在中毒症状、生物测定结果、超微结构水平与RH-5992具有相似的作用。

关键词: 棉铃虫, W200013, RH-5992, 蜕皮, 超微结构

Abstract: Effects of a new type of nosteroidal ecdysone agonist W200013 on the fourth instar larvae of the cotton bollworm (Helicoverpa armigera) were studied by transmission electron microscopy. These observations revealed that W200013 initiated a precocious and lethal larval moult. At 6 h post treatment (pt), the deposition of additional layers of endocuticle, a dramatic increase in rough endoplasmic reticulum and a decrease in glycogen particles were noted in the epidermis. At 12 h pt, the ecdysial space had formed and epidermal cytoplasts appeared “frozen”. At 24 h pt, a double cuticle separated by the ecdysial space had formed and the epidermis was highly vacuolated. At 36 h pt, new epicuticle coated an incomplete new procuticle containing only a small number of lamellae, and the epidermis showed increasing signs of degeneration. As a result, the treated larvae died without normal ecdysis. At the same time, comparison with RH-5992 revealed that the two insect growth regulators produced similar toxic symptoms, bioassay results and ultrastructural changes of the integument.

Key words: cotton bollworm, W200013, RH-5992, moulting, ultrastructure