›› 2002, Vol. 45 ›› Issue (6): 759-763.

• 研究论文 • 上一篇    下一篇

利用mtDNA COⅠ基因序列鉴定我国烟粉虱的生物型

罗晨1, 姚远2, 王戎疆2, 阎凤鸣2*, 胡敦孝3, 张芝利   

  • 出版日期:2002-12-20 发布日期:2002-12-20

The use of mitochondrial cytochrome oxidaseI(mt COI)gene sequences for the identification of biotypes of Bemisia tabaci(Gennadius)in China

LUO Chen1, YAO Yuan2, WANG Rong-Jiang2, YAN Feng-Ming2*, HU Dun-Xiao3, ZHANG Zhi-Li1   

  • Online:2002-12-20 Published:2002-12-20

摘要: 利用mtDNA COⅠ基因片段作标记,采用序列分析的方法,从分子生态学的角度研究了近年来在我国暴发危害的烟粉虱Bemisia tabaci 5个种群(北京一品红种群,广州甘蓝种群,西安一品红种群,北京西红柿种群和新疆吐鲁番棉花种群)的生物型。在5个种群的基因片段上,截取与Texas-B型相应的720bp的序列分析,结果表明所测序列中只有2个碱基与Texas-B型不同,序列相似性为99.7%;在西安一品红种群和新疆吐鲁番棉花种群的原序列中截取与Arizona-B型序列相应的423bp片段,分析表明这两个种群与AZB3型属于同一个单倍型。因此,我国烟粉虱5个实验种群的生物型与Texas-B型和Arizona-B型种群为同一生物型“B”。

关键词: 烟粉虱, PCR, mtDNA, COI基因序列, 生物型

Abstract: Mitochondrial cytochrome oxidaseⅠ(mt COⅠ) (~830 bp) gene sequences were utilized as markers to identify biotypes of 5 populations of Bemisia tabaci in China. Of the 720 characters examined for COⅠsequences, only 2 bases exhibited divergence and overall similarity was 99.7%. Examination of 423 characters in populations from Xi'an and Tulufan revealed that these two populations were of the same haplotype as AZB3. The results indicated that all 5 populations were of the B-biotype.

Key words: Bemisia tabaci, PCR, mtDNA, COⅠ sequence, biotype