光活化杀虫剂α-三噻吩的电子自旋共振分析及其对库蚊保护酶系统活性的影响

›› 2003, Vol. 46 ›› Issue (1): 22-26.doi:

光活化杀虫剂α-三噻吩的电子自旋共振分析及其对库蚊保护酶系统活性的影响

蒋志胜¹，尚稚珍¹，万树青²，徐汉虹²，赵善欢²

出版日期:2003-02-20 发布日期:2003-02-20

ESR analysis of a photo-activated insectsicide and its effects on superoxide dismutase,peroxidase and catalase activity in Culex pipiens pallens

JIANG Zhi-Sheng¹, SHANG Zhi-Zhen¹, WAN Shu-Qing², XU Han-Hong², ZHAO Shan-Huan²

Online:2003-02-20 Published:2003-02-20

摘要/Abstract

摘要： 以淡色库蚊Culex pipiens pallens为试材，初步进行了典型光活化杀虫剂α-三噻吩（α-T）的电子自旋共振（ESR）分析，并研究其对昆虫保护酶系统的影响。ESR分析表明，近紫外光和药剂α-T均可促进活性氧自由基浓度升高，且两者具有协同关系，近紫外光能显著促进α-T增加自由基的作用，处理3 h时试虫自由基总浓度增加了99.6%。近紫外光和药剂α-T对保护酶系统的影响随酶的种类而有所不同，但在近紫外光存在的条件下，α-T对超氧物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活体活性均有抑制作用，且光照3 h时，抑制作用最强。

关键词: &alpha, -三噻吩；淡色库蚊； ESR；自由基；酶活性；保护酶系统

Abstract: Preliminary effects of α-terthienyl (α-T), a typical photo-activated insecticide, on active oxygen radicals and endogenous enzyme activity in the protective enzyme system of 4th instar Culex pipiens pallens larvae were studied by ESR analysis. The results indicated that both near ultraviolet radiation and α-T could enhance the amount of active oxygen radicals in 4th instar larvae of C. pipiens pallens, and the effect of α-T could also be increased by exposure to near ultraviolet radiation. The activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in vivo could be inhibited by α-T, maximal inhibition occurring after larvae had been exposed to near ultraviolet radiation for 3 hours, although the effects of such radiation and α-T on endogenous enzyme activity in the protective enzyme system varied between different enzymes.

Key words: α-terthienyl; Culex pipiens pallens; ESR, active oxygen radicals, enzyme activity, protective enzyme system

蒋志胜¹，尚稚珍¹，万树青²，徐汉虹²，赵善欢². 光活化杀虫剂α-三噻吩的电子自旋共振分析及其对库蚊保护酶系统活性的影响[J]. , 2003, 46(1): 22-26.

JIANG Zhi-Sheng¹, SHANG Zhi-Zhen¹, WAN Shu-Qing², XU Han-Hong², ZHAO Shan-Huan². ESR analysis of a photo-activated insectsicide and its effects on superoxide dismutase,peroxidase and catalase activity in Culex pipiens pallens[J]. , 2003, 46(1): 22-26.

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http://www.insect.org.cn/CN/Y2003/V46/I1/22

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