›› 2003, Vol. 46 ›› Issue (3): 288-291.

• 研究论文 • 上一篇    下一篇

甜菜夜蛾对氯氟氰菊酯抗性的表皮穿透机理

刘永杰12, 沈晋良1*   

  • 出版日期:2003-06-20 发布日期:2003-06-20

Cuticular penetration mechanism of resistance to lambdacyhalothrin in Spodoptera exigua(Hübner)

LIU Yong-Jie1, 2, SHEN Jin-Liang1*   

  • Online:2003-06-20 Published:2003-06-20

摘要: 用三种方法测定了采自南京江浦的甜菜夜蛾Spodoptera exigua对氯氟氰菊酯的抗性。结果表明,抗性水平的次序为3龄幼虫点滴法(5 499.5倍)和5龄幼虫点滴法(3 973.2倍)>3龄幼虫浸叶法(1 041.6倍)>5龄幼虫叶片夹毒法(24.7倍),因此该品系触杀毒力的抗性水平至少为胃毒毒力LD50的160倍。用14C标记氯氟氰菊酯测定甜菜夜蛾抗性和敏感品系5龄幼虫表皮穿透率结果表明,处理后8h,抗性品系5龄幼虫的表皮穿透率仅为敏感品系幼虫表皮穿透率的55.5%。证实表皮穿透率的降低是产生抗性的一个重要机理。

关键词: 甜菜夜蛾, 抗药性, 氯氟氰菊酯, 表皮穿透率

Abstract: Resistance to lambda-cyhalothrin in the beet armyworm, Spodoptera exigua (Hübner), collected from Jiangpu County, Nanjing, Jiangsu Province was determined by three bioassay methods. The results indicate that the sequence of resistance levels was topical application for 3rd instar larvae (5 499.5-fold) and 5th instar larvae (3 973.2-fold)>leaf-dip method for 3rd instar larvae (1 041.6-fold)>leafsandwich method for 5th instar larvae (24.7-fold). Therefore, the resistant strain of S. exigua has a resistance level obtained by way of contact toxicity at least 160 times than that of stomach toxicity. After 8 h treatment the results indicate that the rate of uticular penetration of 14C lambda-cyhalothrin in 5th instar larvae of the resistant strain was only about 55.5% of that in the susceptible strain. It is safe to say that delayed cuticular penetration is an important mechanism responsible for this resistance.

Key words: Spodoptera exigua, insecticide resistance, lambdacyhalothrin, cuticular penetration