›› 2003, Vol. 46 ›› Issue (5): 578-583.doi:

• 研究论文 • 上一篇    下一篇

抗性品系棉蚜乙酰胆碱酯酶和羧酸酯酶的变异

李飞,韩召军, 唐波   

  • 出版日期:2003-10-20 发布日期:2003-10-20

Insensitivity of acetylcholinesterase and increased activity of esterase in the resistant cotton aphid, Aphis gossypii Glover

LI Fei, HAN Zhao-Jun,TANG Bo   

  • Online:2003-10-20 Published:2003-10-20
  • Contact: HAN Zhao_Jun

摘要: 用浸叶法测定了采自我国不同地区(泰安、莱阳、南京、北京和安阳)的棉蚜品系Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ对久效磷、甲胺磷、抗蚜威和灭多威等杀虫剂的抗性水平,各棉蚜品系对杀虫剂的抗性依次为Ⅴ>Ⅳ>Ⅲ,Ⅱ>Ⅰ。进一步研究表明,Ⅴ和Ⅳ品系棉蚜乙酰胆碱酯酶对杀虫剂的敏感性显著下降,Ⅱ品系次之,Ⅲ和Ⅰ品系接近于敏感品系。Ⅴ和Ⅳ品系乙酰胆碱酯酶的Km值显著下降,表明酶发生了质的变化。不同棉蚜抗性品系的酯酶(全酯酶和羧酸酯酶)活性均显著升高,其中Ⅲ品系的酯酶活力为Ⅱ品系的2倍。Ⅴ品系羧酸酯酶Km值达2460.4 μmol/L,而Ⅳ品系仅为84.4 μmol/L,该两个品系羧酸酯酶发生了质的变化。研究结果表明,不同抗性程度的棉蚜品系均存在代谢抗性和靶标抗性。低抗水平的棉蚜品系,以代谢抗性为主,靶标抗性为辅;中抗水平的棉蚜品系,抑或由于解毒代谢酶的活性显著增强,也可能由于靶标的敏感性显著下降;而高抗水平的棉蚜品系,依赖于代谢抗性和靶标抗性的联合作用。

关键词: 棉蚜,  抗性, 乙酰胆碱酯酶,  羧酸酯酶,  抑制中浓度,  米氏常数

Abstract: Resistant levels of cotton aphid strains collected from five different districts, Taian, Laiyang, Nanjing, Beijing and Anyang in China, designa ted as strainⅠ,Ⅱ,Ⅲ,Ⅳ and Ⅴ respectively, were determined with leaf-dip bioassay. The results Indicated that strain Ⅴ was the highest resistant while strainⅠ was the lowest resistant to conventional organophosphorus (OP) and carbamate pesticides, such as methamidophos, monocrotophos, pirimicarb and thiodicarb. Generally, the resistant levels of the five resistant strains were: Ⅴ>Ⅳ>Ⅲ,Ⅱ>Ⅰ. Compared with the sensitive strain 81-171B, the sensitivity of acetylcholinesterase (AChE) from strainⅡ, Ⅳ and Ⅴ apparently decreased and that from strainⅠand Ⅲ were relatively sensitive. The apparently decreasing of Km value indicated that AChE from strain Ⅳ and Ⅴ have been changed in quality. Esterase (total esterase and carboxylesterase) activity increased dramatically in different resistant strains. Esterase activity of strain Ⅲ is two times that from stain Ⅱ. Specially, Km value of carboxylesterase from strain Ⅴ reached 2460.4 μmol/L and that from strain Ⅳ decreased to 84.4 μmol/L It could be deduced that both increased esterase activity and reduced target sensitivity existed in all resistant strains. For lowlevel resistant cotton aphid strain, increased esterase activity plays a much more important role. However, either apparently increased esterase activity or reduced AChE activity in cotton aphid may result in middle-level resistance. Both dramatically increasing of esterase activity and reduction of AChE sensitivity are presented in high-level resistant strains of cotton aphid.

Key words: Aphis gossypii; resistance, acetylcholinesterase, carboxylesterase, median inhibitory concentration, Michaelis constant