棉蚜抗吡虫啉品系和敏感品系主要解毒酶活性比较

›› 2003, Vol. 46 ›› Issue (6): 793-796.

棉蚜抗吡虫啉品系和敏感品系主要解毒酶活性比较

潘文亮，党志红，高占林

出版日期:2003-12-20 发布日期:2003-12-20

Comparison of activities of detoxic enzymes in the imidaclopridresistant and s usceptible strains of the cotton aphid, Aphis gossypii

PAN Wen-Liang，DANG Zhi-Hong，GAO Zhan-Lin

Online:2003-12-20 Published:2003-12-20

摘要/Abstract

摘要： 通过生物测定和生物化学方法比较了棉蚜 Aphis gossypii 对吡虫啉抗性（约为7倍）和敏感品系几种主要解毒酶的活性。结果表明：氧化胡椒基丁醚对两个品系均无明显增效作用。抗性品系中羧酸酯酶和谷胱甘肽S-转移酶的比活力均明显高于敏感品系，抗性品系中羧酸酯酶的K_m值也显著高于敏感品系，说明抗性品系羧酸酯酶与底物的亲和力明显高于敏感品系。上述结果证明羧酸酯酶和谷胱甘肽S-转移酶活力增强在棉蚜对吡虫啉的抗性中起重要作用。

关键词: 棉蚜, 吡虫啉, 抗性机理, 解毒酶

Abstract: The activities of two metabolic enzymes in the imidaclopridresistant (about 7fold) and susceptible strains of the cotton aphid, Aphis gossypii were compared by biochemical methods. Piperonyl butoxide (Pb) had no synergism to imidacloprid in both of the strains. Activities of carboxylesterases (CarE) and gluathione Stransferases (GSTs) in the resistant strain were significantly higher than that in the susceptible one. The K_m of CarE in the resistant strain were also significantly higher than in the susceptible strain, which means that the resistant strain had higher affinity of CarE with the substrate. The results showed that the enhancement of activities of CarE and GSTs would be the main metabolic mechanism in the resistance of A. gossypii to imidacloprid.

Key words: Aphis gossypii, imidacloprid, resistance mechanism, metabolic enzyme s

潘文亮，党志红，高占林. 棉蚜抗吡虫啉品系和敏感品系主要解毒酶活性比较[J]. , 2003, 46(6): 793-796.

PAN Wen-Liang，DANG Zhi-Hong，GAO Zhan-Lin. Comparison of activities of detoxic enzymes in the imidaclopridresistant and s usceptible strains of the cotton aphid, Aphis gossypii[J]. , 2003, 46(6): 793-796.

[1]	李路莎, 袁郁斐, 武磊, 陈敏. 不同寄主植物对美国白蛾幼虫取食行为及解毒酶活性的影响[J]. , 2018, 61(2): 232-239.
[2]	王芹芹, 崔丽, 王奇渊, 杨宏宇, 芮昌辉. 棉铃虫对茚虫威的抗性机理: PBO, DEF和DEM的增效作用及解毒酶活性[J]. , 2017, 60(8): 912-919.
[3]	王康, 庞倩, 张文文, 吉挺. 多菌灵亚致死剂量对意大利蜜蜂幼虫生长发育和解毒酶系活性的影响[J]. , 2017, 60(6): 642-649.
[4]	严林, 郭蕊, 李亚娟, 李琳琳, 卫琼茹, 韩云帮, 刘梦瑶, 马秀莲. 枸杞棉蚜形态和生物学特性研究[J]. , 2017, 60(6): 666-680.
[5]	张元臣, 刘向东. 棉蚜溶菌酶基因AgLYS的克隆与表达分析[J]. , 2017, 60(2): 127-135.
[6]	蔚添添, 和静芳, 罗婷婷, 董应波, 李志国, 苏松坤. 吡虫啉大田使用剂量对意大利蜜蜂工蜂学习和记忆的影响[J]. , 2017, 60(11): 1300-1306.
[7]	梁炜博, 向兴, 王学贵, 杨群芳, 罗家栋. 噻虫啉亚致死剂量对斜纹夜蛾解毒酶系活性与生长繁殖的影响[J]. , 2017, 60(10): 1187-1197.
[8]	郅军锐, 田甜, 温娟, 刘勇. 西花蓟马或二斑叶螨为害的菜豆对两者间后取食者体内保护酶和解毒酶活性的影响[J]. , 2016, 59(7): 707-715.
[9]	蒋恩顺, 朱毅, 王江勇. 黄荆提取物对棉蚜的生物活性[J]. , 2016, 59(5): 538-545.
[10]	张帅, 马艳, 闵红, 于晓庆, 李娜, 芮昌辉, 高希武. 华北棉区主要害虫抗药性监测与治理技术示范[J]. , 2016, 59(11): 1238-1245.
[11]	崔丽, 张靖, 齐浩亮, 王芹芹, 陆宴辉, 芮昌辉. 我国棉花主产区棉蚜对吡虫啉的抗性监测及抗性机理[J]. , 2016, 59(11): 1246-1253.
[12]	刘佳, 周勇, 朱航, 马海昊, 邓希乐, 周小毛, 柏连阳. 斜纹夜蛾抗药性监测及茚虫威对其解毒代谢酶的影响[J]. , 2016, 59(11): 1254-1262.
[13]	江武军, 何旭江, 王子龙, 颜伟玉, 曾志将, 吴小波. 中华蜜蜂细胞色素CYP9E2基因克隆及其表达分析[J]. , 2016, 59(10): 1050-1057.
[14]	阿力甫·那思尔, 艾山·阿布都热依木, 孟玲, 李保平. 不同虫态及虫龄大草蛉与七星瓢虫之间的集团内互作[J]. , 2015, 58(9): 997-1004.
[15]	余慧灵, 向兴, 袁贵鑫, 陈羿渠, 王学贵. 溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响[J]. , 2015, 58(6): 634-641.