›› 2004, Vol. 47 ›› Issue (1): 117-123.

• 研究论文 • 上一篇    下一篇

松毛虫质型多角体病毒的宿主域与交叉感染

赵同海1, 陈昌洁1, 徐静2, 张青文2   

  • 出版日期:2004-02-20 发布日期:2004-02-20

Host range and cross infection of cytoplasmic polyhedrosis viruses from Dendrolimus spp.

ZHAO Tong-Hai1, CHEN Chang-Jie1, XU Jing2, ZHANG Qing-Wen2   

  • Online:2004-02-20 Published:2004-02-20

摘要: 自1956年从赤松毛虫Dendrolimus spectabilis上首次发现赤松毛虫质型多角体病毒1型(D. spectabilis cytovirus 1,DsCPV-1)以来,先后从马尾松毛虫D. punctatus、油松毛虫D. tabulaeformis、赤松毛虫、德昌松毛虫D. p. tehchangensis、文山松毛虫D. p. Wenshangensis和落叶松毛虫D. superans上发现了质型多角体病毒(cytoplasmic polyhedrosis virus,CPV)。病毒基因组dsRNA电泳图谱分析表明,这些松毛虫CPV的不同分离株均属于质型多角体病毒1型(cytovirus 1)。这些松毛虫CPV病毒可以感染鳞翅目10科35种昆虫,其中对多种昆虫具有很高的感染力和良好的杀虫效果,可以从中筛选替代宿主生产松毛虫CPV杀虫剂,用于害虫生物防治。松毛虫CPV接种某些昆虫后病毒的基因组dsRNA电泳图谱发生了改变,可能是异源病毒诱发了宿主自身潜伏型病毒的感染复制。

关键词: 松毛虫, 质型多角体病毒, 宿主域, 交叉感染, 潜伏型病毒

Abstract: Since DsCPV-1 (Dendrolimus spectabilis cytovirus 1) was discovered and separated from Dendrolimus spectabilis in 1965 in Japan, there have been 6 isolates of cytoplasmic polyhedrosis virus separated respectively from D. punctatus, D. tabulaeformis, D. spectabilis, D. p. tehchangensis, D. p. wenshangensis and D. superans in China. Electrophoretic analysis of the genome dsRNA of these CPV isolates showed that they all should be classified into the cytovirus 1 as the DsCPV-1. These Dendrolimus spp. CPV isolates can infect 35 species of insects in 10 families of Lepidoptera and have a well infectious ability and cause high mortality on many of them, so they also could be used in the control and these insects could be used as candidates for alternate hosts to produce Dendrolimus spp. CPV pesticides. The genome profiles of the viruses obtained from some insects infected by these Dendrolimus spp. CPV isolates are different from that of the inoculants, but related closely, if not identical, to viruses from these insects. These may be explained as that the occult viruses of hosts were activated by other viruses.

Key words: Dendrolimus spp., cytoplasmic polyhedrosis virus, host range, cross infection, occult viruses