›› 2004, Vol. 47 ›› Issue (2): 159-165.

• 研究论文 • 上一篇    下一篇

家蚕母性基因的表达序列标签分析

邱咏梅,夏庆友,程道军,沈以红,刘春,林英,查幸福,向仲怀   

  • 出版日期:2004-04-20 发布日期:2004-11-20

Analysis of silkworm maternal genes with expressed sequence tag

QIU Yong-Mei,XIA Qing-You*,CHENG Dao-Jun,SHEN Yi-Hong,LIU Chun,LIN Ying,ZHA Xing-Fu, XIANG Zhong-Huai   

  • Online:2004-04-20 Published:2004-11-20
  • Contact: XIA Qing-You

摘要: 为了研究家蚕Bombyx mori卵中储存的母性遗传信息,以未受精的肾脏型和正常型蚕卵为材料构建了cDNA文库,并对其随机测序,共获得391条表达序列标签(EST)序列,经拼接后得到374条非重复序列。生物信息学分析发现,172个非重复序列与国际数据库GenBank和silkbase中的已知基因具有较高的相似性。除3个已知母性基因外,其余主要与DNA复制、能量代谢、信号转导、转录、蛋白质结构以及胚胎发育中个体形成等相关。该结果可为进一步研究家蚕胚胎早期发育的调控提供基础数据。

关键词: 家蚕, 表达序列标签, 未受精卵, 胚胎发育, 母性基因

Abstract: The cDNA libraries were constructed from the kidney and normal unfertilized eggs of the silkworm, Bombyx mori and sequenced at random in order to study the maternal genetic information contained in the silkworm's eggs. We had successfully sequenced 391 ESTs (expressed sequence tag) and obtained 374 unigenes after assembling. Bioinformatics analysis indicated 172 unigenes had significant similarity with the known genes recorded in GenBank and silkbase. Except three known maternal genes, the rest were mainly related to replication, metabolism,signal transduction, transcription factor, protein structure and embryo development. These results will provide source for further study on the development regulation of early embryo of the silkworm.

Key words: Silkworm, expressed sequence tag, unfertilized eggs, embryo development, maternal gene