检测SDS-聚丙烯酰胺凝胶电泳中家蝇蛋白的新方法——海波银染法

›› 2004, Vol. 47 ›› Issue (2): 273-276.

检测SDS-聚丙烯酰胺凝胶电泳中家蝇蛋白的新方法——海波银染法

安春菊，耿华，李德森，杜荣骞

出版日期:2004-04-20 发布日期:2004-11-20

A new silver staining method for proteins of housefly in sodium dodecyl sulfate-polyacrylamide gel electrophoresis

AN Chun-Ju, GENG Hua, LI De-Sen, DU Rong-Qian

Online:2004-04-20 Published:2004-11-20

摘要/Abstract

摘要： 介绍一种检测SDS聚丙烯酰胺凝胶电泳中家蝇幼虫蛋白的新方法-海波银染法。该方法对传统银染方法中的试剂与步骤加以改进，省略了乙醇固定与洗涤步骤，只需20 min即可完成全部染色过程，且仅在国产分析纯试剂及普通操作条件下，灵敏度可达毫微克级水平。

关键词: SDS-聚丙烯酰胺凝胶电泳, 考马斯亮蓝染色, 蛋白质银染

Abstract: A new Na₂S₂O₄ silver staining method is described for detecting the proteins of housefly separated by SDS-PAGE. In this staining method, besides the regents and steps are different from the classical silver staining method, fixation and ethanol washing are not necessary. Only twenty minutes were needed to complete the whole staining process. This method has a low demand for regents and manipulation, but can still reach the sensitivity level of nanogram.

Key words: SDS-PAGE, Coomassie brilliant blue staining, protein silver staining

安春菊，耿华，李德森，杜荣骞. 检测SDS-聚丙烯酰胺凝胶电泳中家蝇蛋白的新方法——海波银染法[J]. , 2004, 47(2): 273-276.

AN Chun-Ju, GENG Hua, LI De-Sen, DU Rong-Qian. A new silver staining method for proteins of housefly in sodium dodecyl sulfate-polyacrylamide gel electrophoresis[J]. , 2004, 47(2): 273-276.