›› 2004, Vol. 47 ›› Issue (3): 293-297.

• 研究论文 • 上一篇    下一篇

多炔类化合物对亚洲玉米螟产卵驱避作用及玉米螟的触角电位反应

万树青, 徐汉虹, 赵善欢,尚稚珍,蒋志胜,刘准   

  • 出版日期:2004-06-20 发布日期:2004-06-20

Oviposition-repelling activity of synthetic polyacetylenes and electroantennogram responses in Ostrinia furnacalis (Lepidoptera: Pyralidae)

WAN Shu-Qing, XU Han-Hong1, ZHAO Shan-Huan1, SHANG Zhi-Zhen2, JIANG Zhi-Sheng2, LIU Zhun2   

  • Online:2004-06-20 Published:2004-06-20

摘要: 以茵陈二炔为结构母体,人工合成了11个多炔类化合物。采用蜡纸接卵法,测定了11 个化合物对亚洲玉米螟Ostrinia furnacalis的产卵驱避活性。结果表明: 当处理浓度为20 μg/cm2时,受试的化合物对亚洲玉米螟的产卵行为具有一定程度的驱避作用,其中化合物9(1-苯基-4-甲基-丁二炔)对亚洲玉米螟产卵驱避作用明显, 调查处理后的3天、4天、 5天和6天,其产卵驱避率分别为85.71%、80.00%、61.27%和62.51%。触角电位测定表明,受试的11个化合物对亚洲玉米螟成虫触角感受器具有刺激作用,其中化合物6和化合物9能强烈地刺激产生高振幅的动作电位。10 mg/mL浓度处理,测得化合物6触角电位相对值, 雌、雄虫分别为68.22% 和106.60%,化合物9分别为199.19% 和220.60%。经回归分析所测11个化合物的产卵驱避活性与触角电位反应相对值,两者呈现一定程度上的相关性。还讨论了合成的多炔类化合物对亚洲玉米螟可能的作用靶标和结构与活性间的关系。

关键词: 多炔类化合物, 亚洲玉米螟, 产卵驱避活性, 触角电位

Abstract: Oviposition-repelling activity of 11 synthetic polyacetylene compounds and electroantennograms (EAGs) responses in the Asian corn borer, Ostrinia f urnacalis were assayed with the method of accepting egg on paper and EAG recording techniques. The results of bioassay indicated that the majority of tested compounds, except compound 1 and 10, could inhibit the oviposition behavior of O. furnacalis, and among these compounds compound 9 (1-phenyl-2,4-hexadiyne) was the most effective one. The inhibiting rate of compound 9 was from 61.27% to 85.71% at 20 μg/cm2 in 6 days of treatment. EAGs of the moths tested indicated that there were no obvious sexual differences to some tested compounds under concentra tion of 10 mg/mL, which could stimulate the receptors of antenna of the insect to produce action potential. Among these compounds, the most stimulating compounds were compound 6 (1-phenyl-4-(3-nitro)-phenyl-1,3-butadiyne) and compound 9: both compounds were significantly stronger in average EAG amplitude than other tested compounds, and the males responded more sensitively than females to the two compounds. The relative EAGs (Sr) of female and male to compound 6 were 68.2 2% and 106.60%, and to compound 9 were 199.19% and 220.60% at 10 mg/mL respectively. The dosage-responses of compounds 6 and 9 on EAGs showed that they were dose-dependent. The regression analysis of the data of two series showed that the value of depolarization amplitude of antenna receptors induced by the tested compounds correlated in some degree with inhibiting activity in behavioral test. Finally, the action target and relationship between structure and activity of polyacetylenes to O. furnacalis are discussed.

Key words: Polyacetylenes, Ostrinia furnacalis, oviposition-repelling activity, electroantennogram responses