›› 2004, Vol. 47 ›› Issue (3): 379-383.

• 研究论文 • 上一篇    下一篇

桃蛀果蛾寄主生物型分化的RAPD分析

徐庆刚1,2, 花保祯1*   

  • 出版日期:2004-06-20 发布日期:2004-06-20

RAPD analysis on the speciation in host races of Carposina sasakii Matsumura (Lepidoptera: Carposinidae)

XU Qing-Gang1,2, HUA Bao-Zhen1   

  • Online:2004-06-20 Published:2004-06-20
  • Contact: HUA Bao-Zhen

摘要: 以山茱萸蛀果蛾Carposina coreana Kim为外群,用44个随机引物对苹果、山楂、桃、杏、枣和酸枣等6种寄主植物上的桃蛀果蛾Carposina sasakii Matsumura进行了RAPD 分析。结果表明,不同寄主上的桃蛀果蛾之间遗传差异出现了较大的分化,桃蛀果蛾6个寄主生物型之间的平均遗传距离在0.040~0.637之间,尤其是杏生物型分化最大。经聚类分析,可将不同寄主上的桃蛀果蛾明显分成两个类群,杏生物型为一群,其余为一群。两个类群间遗传距离为0.465,亚群内部的遗传距离为0.040~0.369。推测杏树上的“桃蛀果蛾 ”已经分化成为不同的物种。

关键词: 桃蛀果蛾, 山茱萸蛀果蛾, 物种分化, 生物型, RAPD

Abstract: With Carposina coreana Kim as the out-group, using 44 random primers,the RAPD analysis was conducted on the host races of the peach fruit borer, Carposina sasakii Matsumura, from 6 different host plants, i.e., apple, hawthorn, peach, apricot, jujube and wild jujube, in Shaanxi Province. The results show that there exists great genetic differentiation between the host races. The genetic distances among the six host races of the peach fruit borer vary from 0.040 to 0.637, with the apricot race differs most markedly from other host races. Based on the clustering analysis, the six host races of the peach fruit borer from the various host plants can be divided into two sub-groups: the apricot race as a sub-group, and the others as another sub-group. The genetic distance is 0.465 between the two subgroups, and 0.040 to 0.369 within a sub-group. It is sugges ted that the apricot race of the peach fruit borer have been so differentiated genetically with the others that it should be considered as a good species.

Key words: Carposina sasakii, Carposina coreana, speciation, biotype, RAPD