›› 2004, Vol. 47 ›› Issue (4): 424-428.

• 研究论文 • 上一篇    下一篇

登革Ⅱ型病毒经白纹伊蚊滞育卵的传递

郭晓霞1, 赵彤言1*, 董言德1, 蒋书楠2, 陆宝麟1   

  • 出版日期:2004-08-20 发布日期:2004-08-20

Transmission of dengue 2 virus by diapausing eggs of Aedes albopictus

GUO Xiao-Xia1, ZHAO Tong-Yan1*, DONG Yan-De1, JIANG Shu-Nan2, LU Bao-Lin1   

  • Online:2004-08-20 Published:2004-08-20
  • Contact: ZHAO Tong-Yan

摘要: 采用C6/36细胞培养分离病毒的方法检测感染登革Ⅱ型病毒的白纹伊蚊Aedes albopictus滞育卵孵化的F1代蚊虫感染率,从第一个生殖营养周期子代蚊虫中未分离到病毒,第二与第三生殖营养周期子代蚊虫最低感染率没有显著性差异(χ2=0.01,P>0.0 5),感染子代的批阳性率为9.1%,最低感染率为1∶330;间接免疫荧光检测结果表明感染登革Ⅱ型病毒的白纹伊蚊滞育卵孵化的子代成蚊能通过叮咬将登革病毒传播给敏感乳鼠。这些研究结果表明登革病毒能在媒介滞育卵内存活并传至子代,子代蚊虫能通过叮咬敏感宿主水平传播病毒。

关键词: 白纹伊蚊, 登革Ⅱ型病毒, 经卵传递, 水平传播

Abstract: Virus isolation in C6/36 cells was used for detection of the infection rates of F1 progeny from diapausing eggs of infected Aedes albopictus. Eggs from three gonotrophic cycles (GC) were collected, and vertical transmission was not observed in the first gonotrophic cycle, while there was no significant difference observed between the infection rates of the GC2 progeny and that of the GC3 progeny (P>0.05). The total positive rate of pools was 9.1%, and the minimum infection rate was 1∶330. The results of indirect fluorescent antibody test showed that F1 adult mosquitoes obtained from diapausing eggs can transmit dengue 2 virus to sensitive mice horizontally through biting. The horizontal transmission rate was 6.1%. These results indicate that dengue 2 can be transmitted vertically even the infected eggs are induced into diapausing, and F1 adult mosquitoes raised from diapausing eggs can transmit dengue 2 virus to sensitive mice horizontally via biting.

Key words: Aedes albopictus, dengue 2 virus, transovarial transmission, horizontal transmission