保幼激素类似物及蜕皮甾类对亚洲玉米螟幼虫酚氧化酶活性的影响

›› 2004, Vol. 47 ›› Issue (5): 562-566.

保幼激素类似物及蜕皮甾类对亚洲玉米螟幼虫酚氧化酶活性的影响

冯从经¹，戴华国²，符文俊^1*

出版日期:2004-10-20 发布日期:2004-10-20

Effect of juvenile hormone analogue and ecdysteroids on the activity of phenoloxidase in larvae of the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae)

FENG Cong-Jing¹, DAI Hua-Guo², FU Wen-Jun^1*

Online:2004-10-20 Published:2004-10-20
Contact: FU Wen-Jun1

摘要/Abstract

摘要： 分别用1 μg/头、0.1 μg/头和0.01 μg/头浓度的保幼激素类似物methoprene（蒙五一五）体外处理亚洲玉米螟5龄幼虫，测定幼虫体壁组织、血清和血细胞溶离物中酚氧化酶的活性。结果表明： 1 μg/头 methoprene处理组和0.1 μg/头处理组幼虫体壁组织中酚氧化酶活性与对照组相比有显著提高（P<0.01），血清和血细胞溶离物中酚氧化酶活性也显著上升（P<0.01）。将含有20-羟基蜕皮酮的人工饲料饲喂亚洲玉米螟5龄幼虫，处理组幼虫体壁组织的酚氧化酶活性下降(P<0.05)，血清和血细胞溶离物中的酚氧化酶活性均低于对照组（P<0.01）。这些结果表明methoprene可以诱导亚洲玉米螟5龄幼虫体内酚氧化酶活性的上升，而20-羟基蜕皮酮则抑制了酚氧化酶的活性。

关键词: 亚洲玉米螟, 保幼激素类似物, 20羟基蜕皮酮, 酚氧化酶

Abstract: The effect of juvenile hormone analogue (JHA), methoprene, and 20hydroxyecdysone (20-E) on the activity of phenoloxidase in 5th instar larvae of the Asian corn borer, Ostrinia furnacalis was investigated. The activities of phenoloxidase in integument, hemolymph and hemocyte lysate of the treated O. furnacalis larvae were determined. The results showed that when larvae were treated with 1 μg methoprene and 0.1 μg methoprene, the activity of henoloxidase in integument of the larvae was higher than that of the control group (P<0.01), and the activities of phenoloxidase in hemolymph and hemocyte lysate also increased significantly (P<0.01). When larvae were reared with the artificial diet supplemented with 20-hydroxyecdysone, the activity of phenoloxidase in the integument of the larvae was lower than that of the control group (P<0.05), and the activities of phenoloxidase in hemolymph and hemocyte lysate decreased significantly (P<0.01). These results suggest that methoprene stimulates the activity of phenoloxidase, while 20-hydroxyecdysone, in contrast, inhibits the activity of phenoloxidase in O. furnacalis larvae.

Key words: Ostrinia furnacalis, juvenile hormone analogue, 20-hydroxyecdysone, phenoloxidase

冯从经¹，戴华国²，符文俊^1*. 保幼激素类似物及蜕皮甾类对亚洲玉米螟幼虫酚氧化酶活性的影响[J]. , 2004, 47(5): 562-566.

FENG Cong-Jing¹, DAI Hua-Guo², FU Wen-Jun^1*. Effect of juvenile hormone analogue and ecdysteroids on the activity of phenoloxidase in larvae of the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae)[J]. , 2004, 47(5): 562-566.

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