›› 2004, Vol. 47 ›› Issue (6): 830-836.

• 研究论文 • 上一篇    下一篇

昆虫钠通道的结构和与击倒抗性有关的基因突变

唐振华1, 袁建忠1, 庄佩君1, 陶黎明2,1   

  • 出版日期:2004-12-20 发布日期:2004-12-20

The structure of sodium channels and gene mutations associated with knockdown resistance in insects

TANG Zhen-Hua1, YUAN Jian-Zhong1, ZHUANG Pei-Jun1, TAO Li-Ming2,1   

  • Online:2004-12-20 Published:2004-12-20

摘要: 击倒抗性(kdr)是指昆虫和其他节肢动物由于它们的神经系统对DDT和拟除虫菊酯类杀虫剂的敏感性降低而引起的抗性。电压敏感的钠通道是DDT和拟除虫菊酯类杀虫剂的主要靶标。已知拟除虫菊酯是通过改变位于神经膜上的这类通道而发挥其杀虫效果的,钠通道基因的点突变是产生kdr抗性的主要原因。40年来kdr抗性一直是重要的研究课题,但近10年来在kdr分子生物学方面取得了很大进展。本文主要综述了1996年以来所取得的新进展,着重于钠通道的结构、在14种害虫中与kdr抗性相关的钠通道基因突变及其氨基酸序列的多态性。这些结果有助于对拟除虫菊酯改变钠通道的功能及其机理作进一步探究。

关键词: 击倒抗性, 电压敏感的钠通道, 拟除虫菊酯, 基因突变

Abstract: Knockdown resistance (kdr) is used to describe resistance to DDT and pyrethroid insecticides that result from reduced sensitivity of the nervous system in insects and other arthropods. The voltage-sensitive sodium channels are important action targets of DDT and pyrethroids. Pyrethroids are known to exert their insecticidal effects by altering the function of these channels, and the point mutations of their genes are the primary cause for producing kdr resistance. Kdr resistance has been the subject of sustained research interest for more than 40 years, but the past decade has witnessed significant advances in the identification of the mechanisms underlying kdr resistance at the molecular level. This paper mainly reviews new advances on molecular biology of kdr resistance obtained since 1996, with emphasis on the structure of voltage-sensitive sodium channel subunit, gene mutations associated with kdr resistance among 14 species of pest arthropods and their amino acid sequence polymorphisms. These results can provide new insight into the mechanisms by which pyrethroids modify the function of voltage sodium channels.

Key words: Knockdown resistance, voltage-sensitive sodium channel, pyrethroid, gene mutations