›› 2005, Vol. 48 ›› Issue (1): 57-60.

• 研究论文 • 上一篇    下一篇

甜菜夜蛾核型多角体病毒对拟澳洲赤眼蜂的影响:种群参数比较和PCR检测

罗开珺,张古忍,古德祥,庞义   

  • 出版日期:2005-03-20 发布日期:2005-02-20

Effect of Spodoptera exigua multicapsid nucleopolyhedrovirus on Trichogramma confusum: Comparison of population parameters and PCR detection

LUO Kai-Jun, ZHANG Gu-Ren, GU De-Xiang, PANG Yi*   

  • Online:2005-03-20 Published:2005-02-20
  • Contact: PANG Yi

摘要: 用生命表方法和PCR检测研究了甜菜夜蛾核型多角体病毒(Spodopteraexiguamulticapsid nucleopolyhedrovirus, SeMNPV)对拟澳洲赤眼蜂Trichogramma confusum Viggiani的影响。结果表明,在25±1℃下,取食含SeMNPV蜂蜜和纯蜂蜜(对照)的两组赤眼蜂雌蜂的寿命、繁殖力、羽化率、产雌率均无显著差异;内禀增长率(rm)分别为0.3524 和0.3418,周限增长率(λ)为1.42和1.41,非常接近;净繁殖率(RO)分别为 124.34和142.57,平均世代历期为13.6和14.5天, 种群倍增时间分别为1.96和2.03天。对5头取食含SeMNPV蜂蜜的雌蜂的子代的PCR 检测结果也均为阴性。因此,结论认为SeMNPV对拟澳洲赤眼蜂成蜂并无明显影响,体内携带病毒的亲代蜂并不能将病毒传给子代。

关键词: 甜菜夜蛾, 核型多角体病毒, 拟澳洲赤眼蜂, 生命表, 种群参数, PCR

Abstract: The effect of(Spodoptera exigua multicapsid nucleopolyhedrovirus,SeMNPV) on Trichogramma confusumViggiani was studied by the methods of life table and PCR. The results showed that there was no significant difference in population parameters ofT. confemales between the treatment with the mixture of SeMNPVhoney and that with honey alone at 25±1℃. For the treatment with the mixture of SeMNPV and the treatment with honey alone at 25±1℃, the intrinsic rate of increase (rm) of T. confusum females was 0.3524 and 0.3418, respectively; the finite rate of increase (λ) 1.42 and 1.41; the net reproduction rate (R0) 124.34 and 142.57; the mean generation time 13.6 and 14.5 days; the population doubling time 1.96 nd 2.03 days The results of PCR detection of the offsprings of 5 females ofT. confusum which ingested the the mixture of SeMNPVhoney were all negative. It was therefore concluded that there was no distinct effect of SeMNPV on T. confusum adults, and T. confusum female adults infected with SeMNPV could not transmit SeMNPV to their offsprings.

Key words: Spodoptera exigua, nucleopolyhedrovirus, Trichogramma confusum, life table, population parameter, PCR 