转Bt基因玉米对甜菜夜蛾幼虫存活和发育的影响

›› 2005, Vol. 48 ›› Issue (2): 214-220.doi:

转Bt基因玉米对甜菜夜蛾幼虫存活和发育的影响

王振营¹，王冬妍^1,2，何康来¹，白树雄¹，刘慧¹，丛斌²

中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室

出版日期:2005-07-10 发布日期:2005-11-20
通讯作者: 王振营

Effects of transgenic corn hybrids expressing Bacillus thuringiensis Cry1Ab toxin on survival and growth of the beet armyworm, Spodoptera exigua (Hübner)

WANG Zhen-Ying¹, WANG Dong-Yan^1,2, HE Kang-Lai¹, BAI Shu-Xiong¹, LIU Hui¹, Cong Bin²

State Key Laboratory for Biologyof Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Online:2005-07-10 Published:2005-11-20
Contact: WANG Zhen-Ying

摘要/Abstract

摘要： 在室内测定了2种转Cry1Ab基因的Bt玉米MON810和Bt11不同组织对甜菜夜蛾 Spodoptera exigua （Hübner）初孵幼虫以及心叶对4龄幼虫存活和发育的影响，在田间比较了甜菜夜蛾幼虫取食Bt 和非Bt玉米雌穗的存活和为害情况。结果表明，转Cry1Ab基因的Bt玉米的不同组织对甜菜夜蛾初孵幼虫都具有明显的杀虫活性，取食Bt玉米心叶、苞叶、籽粒时甜菜夜蛾均在幼虫期死亡；取食MON810和Bt11雄穗的初孵幼虫化蛹率分别为5.2%和2.1%，羽化率为2.1%和1.0%；取食MON810和Bt11花丝的初孵幼虫化蛹率分别为1.0%和2.1%，但不能羽化。4龄幼虫取食MON810玉米心叶的化蛹率与对照差异不显著，而取食Bt11的化蛹率与对照差异显著；取食两种Bt玉米心叶的４龄幼虫化蛹后的雌、雄蛹重和羽化率与对照组差异显著，但蛹期和平均单雌产卵量差异不显著，虽然对照组羽化的成虫平均产卵量高于Bt玉米组。田间接种初孵幼虫10 天后的调查结果表明，在MON810和Bt11玉米花丝上幼虫存活率分别为1.3%和0.3%, 而对照组分别为12.9%和16.2%；MON810和Bt11玉米雌穗被害率分别为18.3%和5.0%，而对照组分别为93.3%和95.0%，均显著低于对照组。

关键词: 甜菜夜蛾, Bt玉米, Cry1Ab 杀虫蛋白, 存活率, 发育历期, 控制效果

Abstract: Effects of transgenic Bacillus thuringiensis corn on survival and growth of the beet armyworm, Spodoptera exigua (Hübner) larvae, were evaluated with laboratory bioassay and field tests. Two Bt corn hybrids of events MON810 and Bt11 expressing Cry1Ab toxin and their non-Bt controls were employed. The results showed that the neonates of the beet armyworm could not survive when fed on whorl leaves, tassels and silks separately of the two Bt corns, in laboratory bioassay. A small number of neonates could survive and develop to pupate when fed on tassels and silks of MON810 and Bt11: the pupation rate is 5.2% and 2.1% with the emergence rate of 2.1% and 1.0% on MON810 and Bt11 tassels; 1.0% and 2.1% larvae pupates without emergence on MON810 and Bt11 silks, respectively. The duration for the larvae from neonate to pupae was 17 d and 28.5 d when fed on silks of MON810 and Bt11, compared with 14.0 d and 16.0 d on the corresponding non-transgenic isogenic controls, respectively. The larvae fed on tassels and silks of Bt corns had significantly lower weights than that fed on non-Bt controls in 7 days after infestation. The weight of the larvae fed on the non-Bt control husks of MON810 and Bt11 was 27.6 and 52.9 times the weights of the larvae fed on MON810 and Bt11, respectively. Development of the beet armyworm larvae fed on Bt corn was significantly delayed. Resistance level of the Bt corn whorl tissues to the 4th instar larvae was low. No significant difference was found in the pupation rate for the 4th instar larvae fed on the whorl leaves of MON810 (71.7%) and its non-Bt control (75.0%), but otherwise for those fed on Bt11 (56.7%) and its non-transgenic hybrid (85.0%). The weights of female and male pupae and the percentage of emergence when the 4th instar larvae fed on the Bt corn were significantly lower than that fed on non-Bt controls. Larval survival rate and the percentage of injured ears when larvae fed on MON810 and Bt11 silks were significantly lower compared with non-Bt controls assayed 10 d after the inoculation of the neonates in the field tests.

Key words: Spodoptera exigua;Bt corn, Cry1Ab toxin, survival rate, developmental period, control efficacy

王振营，王冬妍，何康来，白树雄，刘慧，丛斌. 转Bt基因玉米对甜菜夜蛾幼虫存活和发育的影响[J]. , 2005, 48(2): 214-220.

WANG Zhen-Ying, WANG Dong-Yan, HE Kang-Lai, BAI Shu-Xiong, LIU Hui, Cong Bin. Effects of transgenic corn hybrids expressing Bacillus thuringiensis Cry1Ab toxin on survival and growth of the beet armyworm, Spodoptera exigua (Hübner)[J]. , 2005, 48(2): 214-220.

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