家蚕催青前期胚胎蛋白质双向电泳图谱分析

›› 2005, Vol. 48 ›› Issue (2): 295-300.doi:

家蚕催青前期胚胎蛋白质双向电泳图谱分析

颜新培¹, 钟伯雄^1*, 徐孟奎¹, 梁建设², 沈飞英¹

浙江大学动物科学学院蚕蜂分子生物学实验室

出版日期:2005-07-10 发布日期:2005-11-20
通讯作者: 钟伯雄

Analysis of protein patterns from embryo of silkwormBombyx mori at earlier stage by two dimensional polyacrylamide gel electrophoresis

YAN Xin-Pei¹, ZHONG Bo-Xiong^1*, XU Meng-Kui¹, LIANG Jian-She²,SHEN Fei-Ying¹

Laboratory of Molecular Biology of Silkworm and Honeybee, College of Animal Science, Zhejiang University

Online:2005-07-10 Published:2005-11-20
Contact: ZHONG Bo-Xiong

摘要/Abstract

摘要： 为了探讨家蚕Bombyx mori胚胎蛋白质整体变化，以多化性品种P50为材料，采用蛋白质双向电泳技术及图像分析技术分析了催青前期胚胎（戊₃以前）各个时期蛋白质图谱及其变化情况。研究发现:从临界Ⅱ期（丙_２）胚胎到缩短期(戊₂)胚胎蛋白质双向电泳图谱基本稳定，存在于临界Ⅱ期胚胎的蛋白斑点在催青前期的4个胚胎中消失的个数较少，仅占22.80％，而在催青的最后2个胚胎中消失的蛋白质斑点却占48.18％；在神经沟出现（丁₁）、腹肢突起（丁₂）、上唇突起（戊₁）和缩短期（戊₂）胚胎的双向电泳图谱中能够检测到100个特异蛋白质斑点，这些特异蛋白质斑点大多在随后邻近的胚胎发育中消失，暗示了这些特异蛋白可能与相应胚胎的形体特征发育有关。

关键词: 家蚕, 蛋白质, 双向电泳, 图谱分析, 胚胎发育

Abstract: The proteins during earlier stages of embryo (before the head thorax differentiation embryo) and their changes were analyzed using silkworm Bombyx mori variety P50 as experimental material by two dimensional polyacrylamide gel electrophoresis and image analysis system to analyze the change of total proteins from silkworm embryo. It was discovered that two dimensional polyacrylamide gel electrophoresis patterns of protein from the critical development Ⅱ stage embryo to the shortening stage embryo stayed basically stable, and only a small number of the protein spots existed in the critical development Ⅱ stage embryo disappeared at four earlier embryo stages of incubation, which only accounted for 22.80％, but the protein spots which disappeared at 2 last embryos of incubation accounted for 48.18％; 100 different protein spots from the neural groove appearance, abdominal outgrowth appearance, labrum appearance and shortening stage embryo can be detected and most of these protein spots disappeared in the embryos closely followed. The results suggested these different proteins might relate to the characteristics of corresponding embryo body development.

Key words: Bombyx mori;protein, two dimensional electrophoresis, image analysis, embryo development

颜新培, 钟伯雄, 徐孟奎, 梁建设, 沈飞英. 家蚕催青前期胚胎蛋白质双向电泳图谱分析[J]. , 2005, 48(2): 295-300.

YAN Xin-Pei, ZHONG Bo-Xiong, XU Meng-Kui, LIANG Jian-She,SHEN Fei-Ying. Analysis of protein patterns from embryo of silkwormBombyx mori at earlier stage by two dimensional polyacrylamide gel electrophoresis[J]. , 2005, 48(2): 295-300.

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