›› 2005, Vol. 48 ›› Issue (4): 526-530.

• 研究论文 • 上一篇    下一篇

多炔类化合物对美洲大蠊的触杀活性及对乙酰胆碱酯酶和腺苷三磷酸酶活性的影响

万树青, 徐汉虹,赵善欢,蒋志胜,尚稚珍, 刘准   

  1. 华南农业大学
  • 出版日期:2005-09-07 发布日期:2005-08-20
  • 通讯作者: 万树青

Contact toxicity of polyacetylenes to Periplaneta americana and their effects on AChE and ATPase

WAN Shu-Qing, XU Han-Hong, ZHAO Shan-Huan, JIANG Zhi-Sheng, SHANG Zhi-Zhen, LIU Zhun   

  1. South China Agricultural University
  • Online:2005-09-07 Published:2005-08-20

摘要:

采用药膜法测定了人工合成的11个多炔类化合物对美洲大蠊Periplaneta americana初孵若虫的触杀活性。结果表明,当化合物处理浓度为20 μg/cm2时,致死率达70%以上的有: 化合物2 (1-叔丁基-4-羟甲基-丁二炔)、化合物9 (1-苯甲基-4-甲基-丁二炔)和化合物10 (O-炔丙基硫代磷酸二乙酯)。经毒力测定,化合物9和化合物10的LC50分别为3.91 μg/cm2和1.50 μg/cm2。化合物2、化合物7 (1-苯基-4-邻硝基苯基-丁二炔)和化合物10对美洲大蠊乙酰胆碱酯酶(AChE)具有抑制活性,抑制率分别为12.00%、27.24%和62.22%。化合物2和化合物10对Na+-K+-ATPase具有抑制活性,抑制率分别为44.55%和31.44%;而化合物5 (1-苯基-4-(3,4-亚甲基二氧)苯基-丁二炔)和化合物6 (1-苯基-4-间硝基苯基-丁二炔)对该酶具有激活活性,激活率分别为24.98%和20.99%。化合物2、化合物4 (1-苯基-4-对甲氧基苯基-丁二炔)和化合物7对Ca2+-Mg2+-ATPase具有抑制活性,抑制率分别为49.02%、38.53%和35.32%, 其他化合物对该酶具有激活活性,其中激活活性最高的为化合物5,激活率达81.12%。

关键词: 多炔类化合物, 美洲大蠊, 触杀活性, ATPase

Abstract:

The contact toxicity of 11 synthetic polyacetylenes was tested to newly hatched Periplaneta americana larvae with the method of drug film. The results showed these compounds, 1-t-butyl-4-hydroxymethyldiacetylene, 1-benzyl-4-methyldiacetylene and di-ethyl-2-propargylthiophosphate caused high mortality of over 70% to the larvae at 20 μg/cm2 The LC50 of 1-benzyl-4-methyl diacetylene and di-ethyl-2-propargyl thiophosphate was 3.91 μg/cm2 and 1.50 μg/cm2, respectively. The effect on AChE of the insect indicated that 1-t-butyl-4-hydroxymethyldiacetylene, 1-phenyl-4-o-nitrophenyl-diacetylene and di-ethyl-2-propargylthiophosphate inhibited its activity, and the inhibition rate was 12.00%, 27.24% and 62.22%, respectively. To ATPase, 1-t-butyl-4-hydroxymethyl diacetylene and di-ethyl-2-propargyl thiophosphate inhibited the activity of Na+K+ATPase, and the inhibition rate was 44.55% and 31.44%, respectively; but 1-phenyl-4-(3,4methylenedioxy)-phenyl-diacetylene and 1-phenyl-4-m-nitrophenyl-diacetylene enhanced the activity of the enzyme, and the activation rate was 24.98% and 20.61%; 1-t-butyl-4-hydroxymethyl-diacetylene,1-phenyl-4-p-methoxyphenyldiacetylene and 1-phenyl-4-o-nitrophenyl-diacetylene inhibited the activity of Ca2+Mg2+ATPase, and the inhibition rate was 49.02%, 38.53% and 35.32%, respectively; but other compounds enhanced activity of the enzyme, among them the highest activity was observed with 1-phenyl-4-(3,4-methylenedioxy)-phenyl-diacetylene, and the activation rate was 81.12%.

Key words: Polyacetylenes, Periplaneta americana, contact toxicity, AChE, Na+K+ATPase, Ca2+Mg2+ATPase