不同温湿度下球孢白僵菌对小猿叶甲的致病力

›› 2005, Vol. 48 ›› Issue (5): 679-686.

不同温湿度下球孢白僵菌对小猿叶甲的致病力

何余容, 邝灼彬, 冯夏, 陈焕瑜, 武亚静

华南农业大学昆虫生态室

出版日期:2005-11-16 发布日期:2005-10-20
通讯作者: 吕利华

Effect of temperature and humidity on the virulence of beetlederived Beauveria bassiana（Balsamo）Vuillemin (Deuteromycetes: Moniliales) against the daikon leaf beetle, Phaedon brassicae Baly (Coleoptera: Chrysomelidae)

HE Yu-Rong, LU Li-Hua, KUANG Zhuo-Bin, FENG Xia, CHEN Huan-Yu, WU Ya-Jing

South China Agricultural University

Online:2005-11-16 Published:2005-10-20

摘要/Abstract

摘要：

本文在室内研究了分离自小猿叶甲的一株球孢白僵菌(SCAU-BB01D)在不同温度和湿度条件下对小猿叶甲成虫和2龄幼虫的致病力。结果显示温度和相对湿度对白僵菌的致病力有显著影响。在17℃和29℃，小猿叶甲成虫和2龄幼虫的死亡率显著低于其他温度; 在23℃下，成虫接菌后第14天的累计死亡率达到最高为95.60%; 在23℃和26℃下，2龄幼虫接菌后第10天达到最高累计死亡率96%。随着相对湿度的提高，球孢白僵菌对小猿叶甲的致病力显著增加，当相对湿度从50%增加至100%时，成虫第14天的累计死亡率从55.70%增加到88.80%，2龄幼虫第10天的累计死亡率从62.00%增加到96.00%。机率值分析结果表明，在17、20、23、26和29℃温度下，白僵菌对小猿叶甲成虫和2龄幼虫的LT₅₀分别为 13.63、10.27、8.05、8.87、12.41天和 6.06、5.72、4.90、4.86、6.73天。随着相对湿度的升高，球孢白僵菌对小猿叶甲的致死中时(LT₅₀)呈缩短的趋势，当相对湿度为50%、60%、70%、80%、90%、100％时，成虫和2龄幼虫的LT₅₀分别为12.97、10.97、10.13、9.79、9.14、8.29天和7.68、6.92、5.76、5.57、5.48、4.82天。由实验结果可以得知，23～26℃是球孢白僵菌感染小猿叶甲最适宜的温度范围，环境湿度越高越有利于病原菌侵染，当相对湿度大于90%时，最有利于球孢白僵菌对小猿叶甲的感染。

关键词: 球孢白僵菌, 小猿叶甲, 温度, 湿度, 致病力

Abstract:

In this study, the effect of temperature and humidity on the virulence of a Beauveria bassiana isolate, SCAU-BB01D, to Phaedon brassicae, a serious insect pest of cruciferous vegetables in South China, was studied in the laboratory. The mortality of P. brassicae was significant lower at temperature of 17℃ and 29℃ than at other temperatures. At temperature of 23℃, the mortality of adult on the 14th day after treatment reached its highest, 95.60%. For 2nd instar larvae the highest mortality (96.00%) was obtained at both 23℃ and 26℃ on the 10th day after treatment. The pathogenicity of B. bassiana to P. brassicae was enhanced as relative humidity increased. When relative humidity (RH) was increased from 50% to 100%, adult mortality 14 days after treatment was enhanced from 55.70% to 88.80% and 2nd instar larvae mortality 10 days after treatment was enhanced from 62.00% to 96.00%. At 17, 20, 23, 26, and 29℃, the LT₅₀ values were 13.63, 10.27, 8.05, 8.87 and 12.41 days for adults, and 6.06, 5.72, 4.90, 4.86 and 6.73 days for 2nd instar larvae, respectively. LT₅₀ values at RH of 50%, 60%, 70%, 80%, 90% and 100% were 12.97, 10.97, 10.13, 9.79, 9.14, 8.29 for adults and 7.68, 6.92, 5.76, 5.57, 5.48, and 4.82 days for 2nd instar larvae, respectively. Therefore, temperatures between 23-26℃ and RH higher than 80% were the most suitable conditions for promoting infection of B. bassiana to P. brassicae.


Key words: Beauveria bassiana, Phaedon brassicae, temperature, humidity, virulence

何余容, 邝灼彬, 冯夏, 陈焕瑜, 武亚静. 不同温湿度下球孢白僵菌对小猿叶甲的致病力[J]. , 2005, 48(5): 679-686.

HE Yu-Rong, LU Li-Hua, KUANG Zhuo-Bin, FENG Xia, CHEN Huan-Yu, WU Ya-Jing. Effect of temperature and humidity on the virulence of beetlederived Beauveria bassiana（Balsamo）Vuillemin (Deuteromycetes: Moniliales) against the daikon leaf beetle, Phaedon brassicae Baly (Coleoptera: Chrysomelidae)[J]. , 2005, 48(5): 679-686.

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