关键词: 柞蚕, 核型多角体病毒, 泛素, 序列分析

Abstract:

Polyhedron particles of Antheraea pernyi nuclear polyhedrosis virus (ApNPV) were purified from A. pernyi pupa. The genomic DNA of ApNPV was extracted, and the viral DNA was digested by HindⅢ and SalⅠ, respectively. A viral fragment with a length 321 base pairs was sequenced, which contains a 76 amino acid residues open reading frame with deductive a molecular weight 8.46 kD, homologous to ubiquitin. By comparing the identity of amino acid sequence of the gene with that of baculoviruses and other organisms, it was found that ApNPV ubiquitin gene had the highest identity (96.1%) with Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpNPV), the second high identity with Autographa californica NPV (AcNPV, 86.8%), and the lowest identity with Adoxophyes orana granulovirus (AoGV, 71.1%), while had 77.6% to 76.3% identity with that of human, nematode and yeast. Although a number of amino acid residues are conservative in the eukaryotic ubiquitin, they are diverse in baculovirus ubiquitin gene. Several amino acid residues are unique to the baculovirus ubiquitin genes.

Key words: Antheraea pernyi, nucleopolyhedrovirus, ubiquitin, sequence analysis