›› 2005, Vol. 48 ›› Issue (6): 876-880.

• 研究论文 • 上一篇    下一篇


胡兆农, 吴文君, 贺秉军,刘安西   

  1. 西北农林科技大学植物保护学院
  • 出版日期:2005-12-29 发布日期:2005-12-20
  • 通讯作者: 胡兆农

Effects of the mixture of celangulin Ⅳ and Ⅴ from Celastrus angulatus Max. on excitatory junction potentials of Drosophila melanogaster larvae

HU Zhao-Nong, WU Wen-Jun, HE Bing-Jun, Liu An-Xi   

  1. College of Plant Protection, Northwest Science and Technology University of Agriculture and Forestry
  • Online:2005-12-29 Published:2005-12-20


利用细胞内微电极记录技术研究了杀虫植物苦皮藤Celastrus angulatus Max.中麻醉成分苦皮藤素Ⅳ和毒杀成分苦皮藤素Ⅴ混合物对果蝇Drosophila melanogaster 3龄幼虫腹纵肌神经肌肉兴奋性接点电位(EJPs)的作用。结果表明,苦皮藤素Ⅳ比例较高(Ⅳ∶Ⅴ= 3∶1)时对EJPs的影响与单用苦皮藤素Ⅳ差不多,而苦皮藤素Ⅴ比例较高(Ⅳ∶Ⅴ= 1∶1和1∶3)时可使EJPs阻断时间明显延长。根据以上结果初步认为,虽然苦皮藤素Ⅳ和Ⅴ单独对EJPs的阻断作用基本相似,但它们对突触谷氨酸受体通道的影响存在明显差异。

关键词: 植物杀虫剂, 苦皮藤素, 混合物, 兴奋性接点电位, 细胞内微电极记录


Celangulin Ⅳ and Ⅴ isolated from the root bark of the Chinese bittersweet, Celastrus angulatus Max. are the major active insecticidal ingredients of this plant. The mixed effects of celangulin Ⅳ and Ⅴ on insect neuromuscular excitatory junction potentials (EJPs) were studied by using the intracellular microelectrode recording on the ventral longitudinal body-wall muscle fibers of the 3rd instar larvae of Drosophila melanogaster. The results indicated that the effects of the mixture of celangulin Ⅳ and Ⅴ on EJPs of D. melanogasterlarvae, in which celangulin Ⅳ was in higher ratio, were same as that of celangulin Ⅳ. The blocking time of EJPs was delayed by the mixture of celangulin Ⅳ and Ⅴ, in which celangulinⅤ was in higher ratio. In conclusion, celangulin Ⅳ and Ⅴ could have different effects on glutamate potential of insect synapses.


Key words: Botanical insecticides, celangulin, mixture, excitatory junction potentials (EJPs), intracellular microelectrode recording