›› 2006, Vol. 49 ›› Issue (3): 521-527.

• 研究论文 • 上一篇    下一篇

基因序列在蚜虫分子系统发育研究中的应用

张合彩, 乔格侠   

  1. 中国科学院动物研究所
  • 出版日期:2006-07-10 发布日期:2006-06-20

Application of gene sequences in molecular phylogenetic study on Aphididae (Hemiptera)

ZHANG He-Cai, QIAO Ge-Xia   

  1. Institute of Zoology, Chinese Academy of Sciences
  • Online:2006-07-10 Published:2006-06-20

摘要:

总结了核基因和线粒体基因在半翅目蚜虫分子系统发育研究中的应用。核基因中EF-1α应用最广泛,适用于探讨属级及属以上的问题; 核rDNA在蚜虫中应用较少,18S rDNA适用于探讨科级以上高级阶元的问题;LWO是新近在蚜虫中开发使用的一个新基因。线粒体基因中,COⅠ/COⅡ使用最多,12S rDNA/16S rDNA、ND1、Cyt b以及F-ATP6均有应用,探讨的问题从属、种级到科级不等。核基因和线粒体基因间以及不同线粒体基因间的联合分析在解决不同层次的问题中均有应用。建议不断尝试新基因以找出适合蚜虫类群的“标准基因”。并对未来蚜虫分子系统发育研究趋势进行了展望。

关键词: 蚜科, 分子系统学, 核基因, 线粒体基因

Abstract:

The application of nuclear and mitochondrial gene sequences in the molecular phylogenetics of Aphididae (Hemiptera) is summarized, with a special focus on the scientific problems which they can resolve. Among the nuclear genes, sequences of EF-1α are used most widely, and they are fit for studying the phylogenies at genus or higher levels; nuclear rDNAs are used less in aphids’ phylogeny analysis, LWO is a new marker developed in aphids, and both of them are applicable for higher-level taxa above family. Among the mitochondrial genes, the most often used markers are COⅠ/COⅡ. 12S rDNA/16S rDNA, ND1, Cyt b and F-ATP6 are also used in Aphidinea phylogeny reconstruction. Combined analyses are performed between nuclear genes and mitochondrial genes, as well as among different mitochondrial genes. New genes should be explored, and “standard markers” need to be found in aphids. The prospects of molecular systematics in Aphidinea in the future are also discussed.

Key words: Aphididae, molecular phylogenetics, nuclear genes, mitochondrial genes