›› 2006, Vol. 49 ›› Issue (6): 1042-1045.

• 研究论文 • 上一篇    下一篇

绿僵菌侵染小菜蛾幼虫过程的透射电镜观察

王音,雷仲仁*,张青文,问锦曾   

  1. (中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100094)
  • 出版日期:2006-12-31 发布日期:2006-12-20
  • 通讯作者: 雷仲仁

Observation of infection process of Metarhizium anisopliae on Plutella xylostella larvae with transmission electron microscopy

WANG Yin, LEI Zhong-Ren, ZHANG Qing-Wen, WEN Jin-Zeng   

  1. (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)
  • Online:2006-12-31 Published:2006-12-20
  • Contact: LEI Zhong-Ren

摘要: 利用透射电镜观察了绿僵菌Metarhizium anisopliae对小菜蛾Plutella xylostella幼虫的侵染过程。结果表明:接种后22 h最早观察到绿僵菌穿透到寄主表皮层中,在穿透表皮过程中伴随着机械力和酶的活动。菌体进入寄主血腔后,以菌丝出芽生殖、菌丝分隔及菌丝段分隔3种方式大量增殖,主要形成颗粒状的菌丝段在寄主体腔内扩散,也形成少量的丝状菌丝。

关键词: 小菜蛾, 绿僵菌, 增殖, 侵染过程, 透射电镜

Abstract: The infection process of two isolates of Metarhizium anisopliae on Plutella xylostella larvae was studied using transmission electron microscopy. The results showed that the penetration of the integument of P. xylostella larva by M. anisopliaewhich was first observed in 22 h after inoculation, was the combination of mechanical pressure and enzymatic action. The penetrant hyphae produced lots of granule hyphal bodies by budding or septation, which became distributed throughout the body cavity of infected larva. Some filar hyphae were also seen in the hemocoel of infected larva.

Key words: Plutella xylostella, Metarhizium anisopliae, propagation, invasion process, transmission electron microscopy