棉铃虫感觉神经元膜蛋白基因克隆和表达

›› 2007, Vol. 50 ›› Issue (1): 7-13.doi:

棉铃虫感觉神经元膜蛋白基因克隆和表达

苏宏华，王桂荣，张永军，梁革梅，吴孔明，郭予元^*

(中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京100094)

出版日期:2007-01-20 发布日期:2007-02-20
通讯作者: 郭予元

Cloning and expression of sensory neuron membrane protein in Helicoverpa armigera.

SU Hong-Hua，WANG Gui-Rong, ZHANG Yong-Jun, LIANG Ge-Mei, WU Kong-Ming, GUO Yu-Yuan

(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)

Online:2007-01-20 Published:2007-02-20
Contact: GUO Yu-Yuan

摘要/Abstract

摘要： 从棉铃虫Helicoverpa armigera触角中克隆了一条全长1 690 bp的cDNA序列，该序列阅读框全长1 572 bp，编码523个氨基酸残基，序列中有2个跨膜区，具有昆虫感觉神经元膜蛋白（sensory neuron membrane protein， SNMP）的典型特征。SNMP与已报道的其他昆虫的感觉神经元蛋白的氨基酸序列有很高的同源性。半定量RT-PCR研究结果显示，SNMP在棉铃虫中不仅在触角中表达，也在去掉触角的头、足中表达。但是在触角中的表达量最高，在雌雄触角中的表达量差异不显著。在喙、下颚须和下唇须中也有表达。SNMP在卵、蛹和成虫体内也都有表达，但在卵中表达量相对较低。将SNMP编码区克隆到表达载体pET21b中，成功地进行了原核表达，表达出带有6个组氨酸标签的重组蛋白。

关键词: 棉铃虫, 感觉神经元膜蛋白, 基因克隆, 半定量RT-PCR, 原核表达

Abstract: The larvae of the Asian corn borer, Ostrinia furnacalis (Guenée), were treated for 2 h in the laboratory under the temperature 5℃ (LT1) and 0℃ (LT2), respectively. The discriminating temperature, survival rate, cold tolerance, super-cooling point, and the rates of water and lipid in the Asian corn borer larvae after low temperature treatment were determined, respectively. The special antifreeze protein in the serum of the 5th instar larvae of O. furnacalis after low temperature treatment was analyzed with SDS-PAGE. The results showed that the discriminating temperatures for the 3rd, 4th and 5th instar larvae were -13.5℃, -16.5℃ and -18.5℃, respectively. The survival rates of 3rd, 4th and 5th instar larvae all showed the tendency of LT2>LT1> the control group after low temperature treatment (P<0.05). With the increase of instar, the cold tolerance of the larvae increased gradually, and the rates of water and lipid in the larvae also increased significantly (P<0.05). The super-cooling point of the larvae was not effected significantly by low temperature (P>0.05). The rates of water and lipid in larval body showed the tendency of LT2>LT1>the control group after low temperature treatment. A special antifreeze protein with the molecular weight about 29.0 kD was detected in the 5th instar larvae. It was so concluded that low temperature treatment could increase the cold tolerance in the Asian corn borer larvae. 19 Refs. In Chinese.

Key words: Helicoverpa armigera, sensory neuron membrane protein, gene cloning, semi-quantitative RT-PCR, prokaryotic expression

苏宏华，王桂荣，张永军，梁革梅，吴孔明，郭予元^*. 棉铃虫感觉神经元膜蛋白基因克隆和表达[J]. , 2007, 50(1): 7-13.

SU Hong-Hua，WANG Gui-Rong, ZHANG Yong-Jun, LIANG Ge-Mei, WU Kong-Ming, GUO Yu-Yuan. Cloning and expression of sensory neuron membrane protein in Helicoverpa armigera.[J]. , 2007, 50(1): 7-13.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/

http://www.insect.org.cn/CN/Y2007/V50/I1/7

[1]	马荣琴, 王林玲, 衡惠, 周泽扬, 李治. 意大利蜜蜂铁蛋白基因AmFer3HCH的克隆及其在温度胁迫下的应答[J]. , 2018, 61(9): 1029-1039.
[2]	单艳敏, 张玉, 霍志家, 庞保平, 孙学涛. 沙葱萤叶甲丝氨酸蛋白酶基因GdSP的克隆及对温度胁迫的响应[J]. , 2018, 61(7): 761-770.
[3]	艾东, 林荣华, 王猛, 梁晓贺, 于彩虹. 棉铃虫可溶型海藻糖酶的化学修饰[J]. , 2018, 61(7): 801-807.
[4]	孙佳斌, 刘乃勇, 李双美, 闫祺, 董双林. 斜纹夜蛾信息素结合蛋白SlitPBP4的分子克隆、组织表达谱及结合特性分析[J]. , 2018, 61(6): 657-667.
[5]	李鹏燕, 刘艳萍, 王思威, 孙海滨, 柏建山, 彭刚, 龚雪海. 荔枝蒂蛀虫信息素结合蛋白基因序列及表达分析[J]. , 2018, 61(5): 527-536.
[6]	司品法, 周琼, 崔中翌 . 柑橘大实蝇气味结合蛋白基因BminOBP25的克隆、原核表达及组织表达分析[J]. , 2018, 61(5): 537-545.
[7]	岳金金, 马宇驰, 刘全, 姜莉莉, 费东亮, 张鹤, 孙莉, 李明, 马鸣潇. 中华蜜蜂半乳糖凝集素AcGalectin的表达、纯化及性质分析[J]. , 2018, 61(5): 546-554.
[8]	程杏安, 林贤伟, 蒋旭红, 刘展眉, 钟国华, 胡美英. 草地贪夜蛾组织蛋白酶L的基因克隆、原核表达及多克隆抗体制备[J]. , 2018, 61(4): 410-422.
[9]	郗学鹏, 魏伟, 张卫星, 王红芳, 胥保华. 中华蜜蜂转录因子AP-1基因的克隆及特性分析[J]. , 2018, 61(4): 423-431.
[10]	陈龙, 谭瑶, 周晓榕, 庞保平, 格希格都仁, 孙志鹏. 沙葱萤叶甲海藻糖酶基因GdTre1的克隆、分子特性和表达分析[J]. , 2018, 61(3): 271-281.
[11]	柳小龙, 张宾, 田浩楷, 宁静, 王海香, 赵莉蔺. 松墨天牛Mfe2基因的克隆、原核表达和表达谱分析[J]. , 2018, 61(3): 282-291.
[12]	李鸿波, 戴长庚, 张昌容, 何永福, 胡阳. 粘虫过氧化氢酶基因的克隆与表达分析[J]. , 2018, 61(2): 178-187.
[13]	申利, 许川, 张蕾, 张奎, 毛景欣, 潘光照, 李重阳, 胡仁建, 杨丽群, 崔红娟. 家蚕C类清道夫受体BmSR-C的基因克隆、原核表达及亚细胞定位[J]. , 2018, 61(10): 1132-1144.
[14]	苗娅, 张甜甜, 许柏英, 陈斌. 中华按蚊谷胱甘肽-S-转移酶E6（AsGSTE6）的表达纯化与结晶[J]. , 2018, 61(1): 59-67.
[15]	谭永安, 赵旭东, 肖留斌, 孙洋, 赵静, 柏立新, 郝德君. 绿盲蝽核受体基因AlE75D的克隆和性质分析[J]. , 2017, 60(9): 984-993.