关键词: 家蚕, 传染性软化病病毒, VP1基因片段, 结构蛋白, 克隆

Abstract: BmIFV-CHN001 is the first strain of infectious flacherie virus isolated in China. In this study, the sequence of VP1 gene fragment coding the main structural protein of BmIFV-CHN001 was amplified. Subsequent cloning and sequencing showed that the sequence fragment was 906 nucleotides in length. Comparison with the VP1 gene of the published Japanese strain indicated that the nucleotide sequence similarity was 99.3% while the amino acid sequence similarity was up to 100%, suggesting that BmIFV-CHN001 was highly homologous to the Japanese strain. Amino acid sequences alignment was performed based on the main structural proteins from BmIFV-CHN001 and other six viruses belonging to the same genus, Iflavirus. A phylogenetic tree was constructed. The results showed that there were close relationships among the seven viruses, while BmIFV-CHN001 had the closest relationship with the sacbrood virus of the honey bee.

Key words: Bombyx mori, infectious flacherie virus, VP1 gene fragment, structural protein, cloning, sequence analysis