关键词: B型烟粉虱, 高温胁迫, 热激蛋白基因hsp70, 表达量, 实时荧光定量RT-PCR, TaqMan-MGB探针

Abstract: The plasmid with hsp70 cDNA of Bemisia tabaci B-biotype was used as a template. Based on the GenBank sequence data, one TaqMan-MGB probe and the corresponding primers were designed to construct a standard for real-time fluorescence quantitative RT-PCR to detect the expression of hsp70 in B. tabaci B-biotype, with which the changes in expression level of hsp70 from adults of B. tabaci B-biotype under different high temperature stress from 37℃ to 45℃ were detected. The results showed that mild high temperature could induce hsp70 expression in B. tabaci B-biotype adults. Within the range of 37℃ to 41℃, the hsp70 expression level of B. tabaci B-biotype adults increased from 8.78×105±6.41×104 copies to 1.99×107±1.45×105 copies; However, when the temperature increased to 43℃ and 45℃, the hsp70 expression level decreased very quickly. The hsp70 expression level of B. tabaci B-biotype adults changed in response to the diurnal temperature change. When air temperature increased from 34℃ to 41℃ in the morning, the hsp70 expression level increased dramatically from 1.16×105±1.48×104 copies to 6.29×106±1.80×105 copies; however, the hsp70 expression level decreased to 2.32×105±7.69×103 copies when air temperature decreased to 33℃ at nightfall. It was so inferred that hsp70 in B. tabaci B-biotype may play an important role in high temperature tolerance.

Key words: Bemisia tabaci B-biotype, high temperature stress, hsp70, expression level, real-time fluorescence quantitative RT-PCR, TaqMan-MGB probe