›› 2007, Vol. 50 ›› Issue (11): 1185-1186.doi:
• 研究论文 • 上一篇 下一篇
刘吉平,曾玲
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摘要: 本研究探讨应用荧光染色试剂Calcofluor White M2R染色鉴别家蚕微孢子虫Nosema bombycis。结果表明:在荧光显微镜下可见家蚕微孢子虫孢子被染上强烈的青蓝色荧光, 而寄主组织碎片、病毒、细菌等不被染色。该法是一种快速有效鉴别微孢子虫的方法。
关键词: 家蚕微孢子虫, Calcofluor White M2R, 荧光染色, 识别技术
Abstract: The fluorescent dye Calcofluor White M2R was applied to stain and identify Nosema bombycis spores. The results showed that the spores were stained in green-blue fluorescence. With this method it is easy to distinguish microsporidian spores from virus, bacteria and fungus spores.
Key words: Nosema bombycis, Calcofluor White M2R, fluorescent staining, diagnosis
刘吉平,曾玲. Calcofluor White M2R荧光染色法识别家蚕微孢子虫[J]. , 2007, 50(11): 1185-1186.
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