›› 2007, Vol. 50 ›› Issue (12): 1304-1308.

• 研究论文 • 上一篇    下一篇

姜黄素类化合物对朱砂叶螨的生物活性

张永强,丁伟,赵志模   

  • 出版日期:2007-12-20 发布日期:2008-01-04
  • 通讯作者: 丁伟

Biological activities of curcuminoids against Tetranychus cinnabarinus Boisduval (Acari: Tetranychidae)

  • Online:2007-12-20 Published:2008-01-04

摘要: 分别用玻片浸渍法和叶片浸渍法测定了从姜黄中分离的姜黄素(curcumin,CCM)、去甲氧基姜黄素(demethoxycurcumin,DMC)和双去甲氧基姜黄素(bisdemethoxycurcumin,BDMC) 3种天然姜黄素类化合物对朱砂叶螨Tetranychus cinnabarinus Boisduval的成螨、若螨、卵的触杀活性以及对成螨产卵的抑制作用。结果表明:3种化合物对朱砂叶螨的生物活性大小依次为BDMC>DMC>CCM。3种化合物对朱砂叶螨成螨触杀活性最高者为BDMC,处理24 h和48 h其LC50分别为1.18和0.51 mg/mL。对若螨触杀活性的大体趋势与对成螨的相同,其中处理48 h,BDMC对若螨的LC50最小,为2.48 mg/mL。3种化合物对朱砂叶螨卵的触杀毒力也同样表现为BDMC>DMC>CCM。3种单体化合物都表现出一定的对朱砂叶螨雌成螨的产卵抑制作用。对姜黄素类化合物构效关系的初步研究,明确了甲氧基在姜黄素模板上对杀螨活性的贡献,对于开发具经济价值的叶螨类杀螨剂或者筛选先导化合物模板,具有一定的参考价值。

关键词: 朱砂叶螨, 姜黄素类化合物, 生物活性

Abstract: The direct contact activities of the curcumin (CCM), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), isolated from the rhizomes of Curcuma longa, against the adults, nymphs and eggs of Tetranychus cinnabarinus Boisduval and oviposition inhibition against female adult mites were examined under the laboratory condition by slide-dip and leaf-dip method, respectively. The results showed that evaluated with the medium lethal concentration (LC50) values, the biological activities of both contact action and oviposition inhibition of three monomers against T. cinnabarinus were in the tendency of BDMC>DMC>CCM. BDMC showed the highest contact toxicity against adult T. cinnabarinus among the three components, whose LC50 values at 24 h and 48 h were 1.18 mg/mL and 0.51 mg/mL, respectively. The general tendency of contact action against T. cinnabarinus nymph was same as against the adult, and the most toxic component to the nymph was BDMC, whose LC50 at 48 h was 2.48 mg/mL. The contact toxicity of curcuminoids against T. cinnabarinus egg also exhibited the tendency of BDMC>DMC>CCM. All the three components showed certain oviposition inhibition activity against T. cinnabarinus female adults. Through the primary structure-activity relationship analysis of curcuminoids, the attribution of methyloxy to acaricidal activities was determined, which could provide valuable information for developing potential new phytophagous acaricide or lead compound.

Key words: Tetranychus cinnabarinus, curcuminoids, biological activity