›› 2007, Vol. 50 ›› Issue (2): 186-190.

• 研究论文 • 上一篇    下一篇

脱氧鬼臼毒素对粘虫几种代谢酶系的影响

马志卿,李广泽,冯俊涛*,张兴   

  1. (西北农林科技大学无公害农药研究服务中心,陕西杨凌712100)
  • 出版日期:2007-03-08 发布日期:2007-11-20
  • 通讯作者: 冯俊涛

Effects of deoxypodophyllotoxin on metabolizing enzymes in Mythimna separata Walker

MA Zhi-Qing, LI Guang-Ze, FENG Jun-Tao, ZHANG Xing   

  1. (Biorational Pesticide Research and Development Center, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100,China)
  • Online:2007-03-08 Published:2007-11-20
  • Contact: FENG Jun-Tao

摘要: 脱氧鬼臼毒素是砂地柏Sabina vulgaris Ant.中的主要杀虫活性成分之一。为探讨其作用机理,以叶碟饲喂法处理粘虫Mythimna separata Walker 4龄幼虫,测定了饲喂处理12h、24 h、36 h、48 h和72 h后试虫的羧酸酯酶(CarE)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(AKP)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFO)的活性。结果表明:脱氧鬼臼毒素对粘虫羧酸酯酶的活性无明显影响;对酸性磷酸酯酶具有明显的抑制作用,且随着处理时间的延长,抑制作用增强;对碱性磷酸酯酶的影响较为复杂,表现为先抑制,后有所恢复,再被抑制的变化过程;对谷胱甘肽S-转移酶处理12 h后活性变化不明显,24 h后被抑制,36 h后逐渐激活,与对照差异极显著;对细胞色素P450酶系的O-脱甲基酶活性具有明显的抑制作用。

关键词: 粘虫, 砂地柏, 脱氧鬼臼毒素, 代谢酶, 抑制作用

Abstract: Deoxypodophyllotoxin is the main insecticidal composition in Sabina vulgaris Ant., an insecticidal plant. In order to study its insecticidal mechanism, the effect of deoxypodophyllotoxin on activities of carboxyl esterases (CarE), phosphatase, glutathione S-transferase (GSTs) and microsomal mixed function oxidase (MFO) in larvae of Mythimna separata Walker were assayed. The results showed that the activities of CarE in tested larvae had no distinct change; with extending of treatment time, the activities of acid phosphatase (ACP) in tested larvae were gradually inhibited by deoxypodophyllotoxin; the change of alkaline phosphatase (AKP) activity was very complex; the activity of GSTs had no distinct change in 12 h, was inhibited in 24 h, but was activated in 36 h; the O-demethylase activity of cytochrome P450 was inhibited by deoxypodophyllotoxin.

Key words: Mythimna separata, Sabina vulgaris, deoxypodophyllotoxin, metabolizing enzymes, inhibition