烟粉虱对拟除虫菊酯杀虫剂的抗性机理

›› 2007, Vol. 50 ›› Issue (3): 241-247.

烟粉虱对拟除虫菊酯杀虫剂的抗性机理

何玉仙，黄建，杨秀娟，翁启勇^*

（福建省农业科学院植物保护研究所，福州350013）

出版日期:2007-03-20 发布日期:2007-06-20
通讯作者: 翁启勇

Pyrethroid resistance mechanisms in Bemisia tabaci（Gennadius）

HE Yu-Xian, HUANG Jian, YANG Xiu-Juan, WENG Qi-Yong

(Institute of Plant Protection，Fujian Academy of Agricultural Sciences，Fuzhou 350013)

Online:2007-03-20 Published:2007-06-20
Contact: WENG Qi-Yong

摘要/Abstract

摘要： 通过增效剂生物测定、生化分析以及钠离子通道基因ⅡS4-6 cDNA片段的RT-PCR扩增，探讨了烟粉虱Bemisia tabaci（Gennadius）对拟除虫菊酯杀虫剂的抗性机理。结果表明：对于采自田间的6个烟粉虱抗性品系，磷酸三苯酯（TPP）和胡椒基丁醚（PBO）对氯氰菊酯、溴氰菊酯、氯氟氰菊酯和甲氰菊酯均有显著的增效作用，而DEM对4种拟除虫菊酯杀虫剂均无明显的增效作用。烟粉虱抗性品系的α-NA羧酸酯酶和β-NA羧酸酯酶活性分别是敏感品系的2.16～2.65倍和1.22～1.41倍，抗性品系的谷胱甘肽S转移酶活性与敏感品系没有差异，表明羧酸酯酶和多功能氧化酶在烟粉虱对拟除虫菊酯类杀虫剂的抗性中具有重要的作用，而谷胱甘肽S转移酶与抗性无关。通过RT-PCR克隆了6个烟粉虱田间抗性品系的钠离子通道结构域ⅡS4-6 cDNA片段的序列（420 bp），发现与敏感品系相比，有2个位点发生突变，分别为L925I突变和I917V突变，L925I突变在所有6个烟粉虱田间抗性种群中均有发生，该位点突变已被证实与拟除虫菊酯类杀虫剂密切相关，表明神经不敏感性可能是烟粉虱对拟除虫菊酯产生抗性的另一个重要因子。

关键词: 烟粉虱, 抗药性, 拟除虫菊酯类杀虫剂, 抗药性机理

Abstract: Resistance mechanisms to pyrethroids were investigated in Bemisia tabaci （Gennadius）with synergist bioassay, biochemical analysis and RT-PCR amplification for ⅡS4-6 region of sodium channel gene. Significant synergisms by PBO and TPP but no obvious synergism by DEM to cypermethrin, deltamethrin, lambda-cyhalothrin and fenpropathrin were found in field-collected resistant strains of B. tabaci. The carboxylesterase （CarE） activities to α-naphthylacetate and β-naphthylacetate in the resistant strains were 2.16－2.65 and 1.22－1.41 times that in the susceptible strain respectively, but there was no difference in the GST activities among the resistant strains and the susceptible strain. This indicated that CarE and mixed function oxidase （MFO） played an important role in the resistance of B. tabaci to pyrethroids, while the resistance of B. tabaci to pyrethroids was not related to GSTs. Nucleotide and deduced amino acid sequences of ⅡS4-6 region of sodium channel in six different field strains of B. tabaci in Fujian province was amplified by reverse transcriptase-mediated polymerase chain reaction analysis （RT-PCR）. Two mutations were identified in theⅡS4-6 linker of the para-type sodium channel of B. tabaci： isoleucine to

valine at position 917（I917V） and leucine to isoleucine at position 925（L925I）. The L925I mutation, occurred in all six different field strains, was identified to be associated with pyrethroid resistance. This suggested that nerve insensitivity might be another mechanism of resistance to pyrethroids in B. tabaci.

Key words: Bemisia tabaci, insecticide resistance, pyrethroids, resistance mechanisms

何玉仙，黄建，杨秀娟，翁启勇^*. 烟粉虱对拟除虫菊酯杀虫剂的抗性机理[J]. , 2007, 50(3): 241-247.

HE Yu-Xian, HUANG Jian, YANG Xiu-Juan, WENG Qi-Yong. Pyrethroid resistance mechanisms in Bemisia tabaci（Gennadius）[J]. , 2007, 50(3): 241-247.

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