绿僵菌素的分离制备及其对蛴螬的毒力

›› 2007, Vol. 50 ›› Issue (5): 461-466.

绿僵菌素的分离制备及其对蛴螬的毒力

胡琼波，任顺祥^*，刘树艳

(华南农业大学资源环境学院，广州 510642)

出版日期:2007-06-08 发布日期:2007-10-20
通讯作者: 任顺祥

Purification of destruxins produced by Metarhizium anisopliae and bioassay of their insecticidal activities against grubs

HU Qiong-Bo, REN Shun-Xiang, LIU Shu-Yan

(College of Natural Resources and Environments, South China Agricultural University, Guangzhou 510642, China)

Online:2007-06-08 Published:2007-10-20
Contact: REN Shun-Xiang

摘要/Abstract

摘要： 以金龟子绿僵菌金龟子变种Metarhizium anisopliae var. anisopliae菌株MaQ10发酵液为材料，采用萃取、浓缩、制备色谱及重结晶技术，分离纯化出5种绿僵菌素的晶体，经与标准样品以及参考文献相对照，此5种晶体与绿僵菌素A、A2、B、C和E相吻合。进而，采用浸渍法测定了绿僵菌素A和B对大等鳃金龟Exolontha serrulata (Gyllenhall) 和卵圆齿爪鳃金龟Holotrichia ovata Chang 1龄幼虫的触杀毒力。结果表明：绿僵菌素A和B对大等鳃金龟的触杀活性，在处理后96～120 h内最高，LC₅₀分别为78.1571 mg/L和88.7562 mg/L；处理浓度为300 mg/L时，LT₅₀分别为13.4159 h和10.5331 h。绿僵菌素A和B对卵圆齿爪鳃金龟的触杀活性，在处理后96 h时最高，LC₅₀分别为66.5308 mg/L和79.4309 mg/L；处理浓度为300 mg/L时，LT₅₀分别是13.6399 h和9.9451 h。

关键词: 绿僵菌素, 分离制备, 毒力, 大等鳃金龟, 卵圆齿爪鳃金龟

Abstract: Five crystal destruxins were purified from the fermentation broth of Metarhizium anisopliae var. anisopliae strain MaQ10 via preparative HPLC and re-crystalization, which were classified to destruxin A, B, C, E and A₂, respectively. Contact toxicities of destruxins A and B against the grubs Exolontha serrulata (Gyllenhall) and Holotrichia ovata Chang were bioassayed by immersing the insects in aqueous dilutions. The results indicated that at 96 and 120 h after treatment, the LC₅₀s of destruxins A and B were estimated as 78.1571 mg/L and 88.7562 mg/L towards E. serrulata and 66.5308 mg/L and 79.4309 mg/L towards H. ovata, respectively. At the concentration of 300 mg/L, the LT₅₀s of destruxins A and B were 13.4159 h and 10.5331 h towards E. serrulata, and 13.6399 h and 9.9451 h towards H. ovata, respectively.

Key words: Metarhizium anisopliae destruxins, purification, toxicity, Exolontha serrulata, Holotrichia ovata

胡琼波，任顺祥^*，刘树艳. 绿僵菌素的分离制备及其对蛴螬的毒力[J]. , 2007, 50(5): 461-466.

HU Qiong-Bo, REN Shun-Xiang, LIU Shu-Yan. Purification of destruxins produced by Metarhizium anisopliae and bioassay of their insecticidal activities against grubs[J]. , 2007, 50(5): 461-466.

[1]	李国平, 封洪强, 黄博, 金银利, 田彩红, 黄建荣, 邱峰. 应用瓶膜法和人工饲料混合法测定比较16种杀虫剂对绿盲蝽的毒力(英文)[J]. , 2017, 60(6): 650-658.
[2]	张龙娃, 康克, 刘玉军, 张晶, 孙龙, 詹成,黄长春, 蒋丽雅, 叶开云, 丁德贵. 美国白蛾高毒力球孢白僵菌菌株筛选[J]. , 2016, 59(1): 111-118.
[3]	刘霞，王红艳，夏晓明，乔康，王开运. 氰虫酰胺对小菜蛾生物学特性及相关酶活性的影响(英文）[J]. , 2014, 57(7): 815-823.
[4]	杨振国, 张永强, 丁伟, 罗金香, 秦培元. 东莨菪内酯与双脱甲氧基姜黄素对朱砂叶螨毒力的温度效应[J]. , 2012, 55(4): 420-425.
[5]	李茂业，林华峰，李世广，金立. 黄绿绿僵菌Mf82菌株对不同虫态褐飞虱的毒力[J]. , 2012, 55(3): 316-323.
[6]	Neda NABAEI, Ali MEHRVAR, Moosa SABER, Mohammad BAGHERI. 昆虫病原真菌与硅藻土混用对四纹豆象的毒力效果(英文)[J]. , 2012, 55(11): 1282-1288.
[7]	魏纪珍, 郭予元, 高希武, 张涛, 梁革梅. Cry1Fa对Cry1Ac抗性棉铃虫的毒力评价[J]. , 2012, 55(10): 1154-1160.
[8]	杨洪, 王召, 金道超. 氯虫苯甲酰胺对白背飞虱实验种群的亚致死效应[J]. , 2012, 55(10): 1161-1167.
[9]	孟翔, 胡俊杰, 金丰良, 任顺祥. 绿僵菌素A和B对斜纹夜蛾SL-1细胞的增殖抑制和致凋亡作用[J]. , 2011, 54(9): 1003-1009.
[10]	任学祥, 王刚, 左一鸣, 王开运, 王杰. 噻虫胺对桃蚜的毒力及其亚致死剂量对桃蚜解毒酶系活力的影响[J]. , 2011, 54(3): 299-305.
[11]	郭天娥，张正群，周超，刘峰，慕卫. 山东省五个棉花产区绿盲蝽对药剂的敏感性检测（英文）[J]. , 2010, 53(9): 993-1000.
[12]	刘雨晴，薛明，张庆臣，周方园，尉吉乾. 黄荆中β-石竹烯对棉蚜的毒力和作用机理[J]. , 2010, 53(4): 396-404.
[13]	尹飞, 胡琼波, 钟国华, 胡美英. 绿僵菌素对爪哇棒孢霉SP053菌株的影响及其混用对小菜蛾的联合毒力[J]. , 2010, 53(1): 61-67.
[14]	张正群, 郭天娥, 王伟, 刘峰, 慕卫. 两种室内生物测定方法评价杀虫剂对绿盲蝽的相对毒力[J]. , 2009, 52(9): 967-973.
[15]	汤强，章玉萍，谢翎，宋礼华，李增智，黄勃. 玫烟色棒束孢几丁质酶的转基因球孢白僵菌菌株的获得及其对马尾松毛虫的毒力增效作用[J]. , 2009, 52(7): 755-763.