细辛醚对粘虫幼虫的毒力及几种重要酶系的影响

›› 2007, Vol. 50 ›› Issue (6): 574-577.doi:

细辛醚对粘虫幼虫的毒力及几种重要酶系的影响

张静，冯岗，马志卿，冯俊涛^*，张兴

(西北农林科技大学无公害农药研究服务中心/陕西省生物农药工程技术研究中心，陕西杨凌712100)

出版日期:2007-06-20 发布日期:2007-12-20
通讯作者: 冯俊涛

Toxicity of sarisan against Mythimna separate Walker and its effects on AChE and ATPases

ZHANG Jing, FENG Gang, MA Zhi-Qing, FENG Jun-Tao, ZHANG Xing

(Research and Development Center of Biorational Pesticide/Shannxi Research Center of Biopesticide Engineering and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China)

Online:2007-06-20 Published:2007-12-20
Contact: FENG Jun-Tao

摘要/Abstract

摘要： 采用点滴法测定了细辛醚对粘虫Mythimna separate Walker 5龄幼虫的触杀毒力，观察了细辛醚对粘虫的致毒症状，并测定了对不同中毒阶段其头部或中肠乙酰胆碱酯酶（AChE）和腺苷三磷酸酶(ATPase)离体活性以及乙酰胆碱（ACh）含量的影响。结果表明：细辛醚对粘虫5龄幼虫12 h的触杀LD₅₀值为106.93 μg/头；中毒粘虫依次表现出兴奋、痉挛、麻痹和死亡等症状，类似于神经毒剂的致毒症状，但试虫从中毒到死亡历期较长，且中毒试虫肠内有气泡产生，此现象异于典型的神经毒剂；对Na+，K+-ATPase有较强的抑制作用，在兴奋期、痉挛期和麻痹期，酶抑制率分别为33.29%、44.45%和23.98%；对AChE活力在不同阶段的抑制率分别是21.64%、29.75%和35.22%；ACh含量有所增加。

关键词: 粘虫, 细辛醚, 钠、钾腺苷三磷酸酶, 乙酰胆碱酯酶, 乙酰胆碱, 酶活性

Abstract: The contact toxicity of sarisan against 5th instar larvae of Mythimna separate Walker was tested under the laboratory conditions with the method of topical application, their toxicity symptoms were observed, and its effects on the in vitro activities of AChE and ATPase and the content of ACh in heads or midguts of the larvae in different stages of toxicity were assayed. The results showed that the contact toxicity LD₅₀ value of sarisan against the 5th instar larvae of M. separate in 12 h post treatment was 106.93 μg/larva. In vivo toxicity symptoms were characterized by a definite sequence of events, starting with excitation, convulsion, paralysis, and death. Hollow duodenum was found in M. separata.AChE and Na+， K+-ATPase in M. separate larvae were strongly inhibited by sarisan, and a higher ACh content was caused by sarisan. During excitation, convulsion and paralysis of treated larvae, the inhibition rates of AChE were 21.64%, 29.75% and 35.22%, respectively, while the inhibition rates of Na+，K+-ATPase were 33.29%, 44.45% and 23.98%, respectively.

Key words: Mythimna separata；sarisan, Na+, K+-ATPase；AChE；ACh；enzyme activity

张静，冯岗，马志卿，冯俊涛^*，张兴. 细辛醚对粘虫幼虫的毒力及几种重要酶系的影响[J]. , 2007, 50(6): 574-577.

ZHANG Jing, FENG Gang, MA Zhi-Qing, FENG Jun-Tao, ZHANG Xing. Toxicity of sarisan against Mythimna separate Walker and its effects on AChE and ATPases[J]. , 2007, 50(6): 574-577.

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