家蚕蛹期雌雄生殖腺蛋白质双向电泳比较分析

›› 2007, Vol. 50 ›› Issue (6): 628-633.doi:

家蚕蛹期雌雄生殖腺蛋白质双向电泳比较分析

毛立明，林健荣^*，赵峰，钟杨生，王叶元，孔庆明，徐秋云

(华南农业大学动物科学学院蚕丝科学系，广州510642)

出版日期:2007-06-20 发布日期:2007-12-20
通讯作者: 林健荣

Comparison of proteins from the pupal gonads of silkworms (Bombyx mori) of both sexes by two dimensional electrophoresis

MAO Li-Ming, LIN Jian-Rong, ZHAO Feng, ZHONG Yang-Sheng, WANG Ye-Yuan, KONG Qing-Ming, XU Qiu-Yun

(Department of Silkworm Science, College of Animal Science, South China Agricultural University, Guangzhou 510642, China)

Online:2007-06-20 Published:2007-12-20
Contact: LIN Jian-Rong

摘要/Abstract

摘要： 分析家蚕Bombyx mori雌雄生殖腺细胞蛋白质，有利于发现性别分化相关的功能蛋白质，探讨生殖腺发育相关基因的表达调控机理。本研究利用蛋白质双向凝胶电泳和图像分析技术，分析家蚕蛹期第2天的雌雄生殖腺细胞蛋白质。结果表明: 在雄蚕生殖腺蛋白质电泳图谱中共检测到435个蛋白斑点，其中特异性蛋白斑点73个，占总蛋白斑点数的16.8％；雌蚕生殖腺的电泳图谱中有417个蛋白斑点，其中特异性蛋白斑点55个，占总蛋白斑点数的13.2％。雌雄能匹配的蛋白斑点有362对，匹配率达85.0％。

关键词: 家蚕, 生殖腺, 蛋白质, 双向电泳, 图谱分析

Abstract: The comparison of protein patterns between male and female gonads will not only contribute to the finding of functional proteins related to sexual traits, but also help exploring the regulating mechanism of related genes during the development of gonad. In this study, using two dimensional electrophoresis (2DE) and gel image analysis system, we compared the proteins in the male and female gonads of silkworm at the 2nd day of the pupal stage. A total of 435 protein spots were detected in the 2-DE pattern of male gonad, of which 73 were the specific protein spots, occupying 16.8% of the total. There were 417 protein spots in the 2-DE pattern of female gonad, of which 55 were specific ones, occupying 13.2% of the total. Comparison showed that 362 pairs were matched in the 2-DE patterns of both male and female, with the match percentage as high as 85.0%.

Key words: Bombyx mori, gonad, protein, 2DE, image analysis

毛立明，林健荣^*，赵峰，钟杨生，王叶元，孔庆明，徐秋云. 家蚕蛹期雌雄生殖腺蛋白质双向电泳比较分析[J]. , 2007, 50(6): 628-633.

MAO Li-Ming, LIN Jian-Rong, ZHAO Feng, ZHONG Yang-Sheng, WANG Ye-Yuan, KONG Qing-Ming, XU Qiu-Yun. Comparison of proteins from the pupal gonads of silkworms (Bombyx mori) of both sexes by two dimensional electrophoresis[J]. , 2007, 50(6): 628-633.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/

http://www.insect.org.cn/CN/Y2007/V50/I6/628

[1]	张祥乐, 马俐, 马倩, 李胜, 刘素宁. Ras信号通路通过激活转录因子Myc促进核内复制细胞生长[J]. , 2018, 61(8): 885-894.
[2]	孟竹, 文茂羽, 康晓丽, 卢忠燕, 赵萍, 夏庆友. 饥饿胁迫对家蚕糖脂代谢的影响及BmFoxO的作用[J]. , 2018, 61(8): 895-904.
[3]	殷娅茹, 胡建, 胡文波, 杨成飞, 王坤, 刘春, 林英, 朱勇, 王凌燕. 家蚕油蚕oc突变体突变基因的精细定位[J]. , 2018, 61(6): 627-636.
[4]	王欢, 徐希莲. 熊蜂营养与饲料研究进展[J]. , 2018, 61(5): 620-626.
[5]	朱娟, 谢雨辰, 陈艳荣, 唐顺明, 易咏竹, 沈兴家. 家蚕滞育关联山梨醇脱氢酶基因的启动子活性分析[J]. , 2018, 61(4): 391-397.
[6]	龙定沛, 郝占章, 向仲怀, 赵爱春. 家蚕基因组靶向编辑技术研究进展 [J]. , 2018, 61(3): 371-384.
[7]	申利, 许川, 张蕾, 张奎, 毛景欣, 潘光照, 李重阳, 胡仁建, 杨丽群, 崔红娟. 家蚕C类清道夫受体BmSR-C的基因克隆、原核表达及亚细胞定位[J]. , 2018, 61(10): 1132-1144.
[8]	杨娟娟, 王远卓, 魏博帆, 邢秋婷, 阚云超, 乔惠丽. 家蚕miR-301对化学感受蛋白基因csp9表达的调控[J]. , 2018, 61(10): 1145-1152.
[9]	唐芬芬, 张永红, 邵榆岚, 朱峰, 白兴荣. 云南家蚕核型多角体病毒分离株的毒力测定及系统进化分析[J]. , 2018, 61(10): 1160-1169.
[10]	马行洲, 李飞. miRNA调控昆虫精巢干细胞自我更新相关基因的保守性分析和靶标验证[J]. , 2017, 60(8): 847-856.
[11]	庞倩, 沈芳, 王康, 张文文, 殷玲, 吉挺. 不同龄期移虫发育的蜂王卵巢蛋白质组学分析(英文)[J]. , 2017, 60(7): 760-771.
[12]	崔乐, 程瑞, 姜楠. 中国无蚬蛾科分布——“蒲公英蚬蛾”实为一种蚕蛾[J]. , 2017, 60(6): 731-734.
[13]	唐芬芬, 郑亚强, 白兴荣, 陈斌. 短时热激后家蚕对核型多角体病毒的抗性及相关抗菌肽和热激蛋白基因表达水平的变化[J]. , 2017, 60(5): 505-512.
[14]	杨基贵, 彭丽娜, 刘太行, 吴云飞, 杜国玉, 张倩, 王炜, 潘敏慧. 家蚕Sox家族基因BmDichaete的克隆与表达分析[J]. , 2017, 60(5): 513-522.
[15]	廖文丽, 卜晓玲, 江婉仪, 刘吉升. 脂多糖对家蚕幼虫Toll样受体基因BmToll9-2表达的影响[J]. , 2017, 60(4): 372-378.