关键词: 光滑鳖甲, 热休克蛋白70, 克隆, 表达分析, 温度胁迫, 半定量RT-PCR

Abstract: The heat shock protein 70 gene (hsp70) from Anatolica polita borealis (a desert beetle distributed in Xinjiang) was isolated through PCR and RT-PCR technique. Sequence analysis indicated that the APhsp70 gene (GenBank accession no. EF569673) has 2 092 bp in length, includes one full open reading fragment and potentially encodes a 653 amino acid residuum protein. The protein deduced from the isolated gene had a calculated molecular mass of 70 kD and was therefore named APHSP70. The APhsp70 gene contains no intron. The expression pattern of APhsp70 was examined at the transcription level after treatment with heat and cold stress. The Northern blot and semi-quantitative RT-PCR results revealed that the APhsp70 was an inducible 70 kDa isoform whose expression was specifically induced by the different stress factors.