关键词: 小地老虎, 触角电位, 性信息素腺体浸提液, 标准化合物

Abstract: The EAG responses of the male black cutworm moth, Agrotis ypsilon (Rottemberg) to standard chemicals of sex pheromone, i.e, Z7-12：Ac (A), Z9-14：Ac (B), Z11-16：Ac (C), Z5-10：Ac (D) and Z8-12：Ac (E), were measured. The results showed that all the standard chemicals tested could elicit the EAG responses. Among the five components, component A (Z7-12：Ac) elicited the highest EAG response (5.65 mV), B (Z9-14：Ac) and C (Z11-16：Ac) elicited the second highest EAG, while D (Z5-10：Ac) elicited the lowest EAG (2.50 mV). The binary compounds, trinary compounds, four components compounds and full components compounds were also able to elicit higher EAG response, which were significantly higher than that of single component. The trinary compound ABC could elicit the highest EAG response, equal to that by the extracts of 5 female sex pheromone glands. The dose-response test of male antenna to each component and the compounds of sex pheromone took on the “S" type approximately with the increase of dose from 0.01 ng to 100 μg. From the response of male moths of different age to standard chemicals, we found that the response value reached the peak on the third day, and then reduced with the increase of age.

Key words: Agrotis ypsilon, electroantennogram, sex pheromone extracts, standard chemicals