丽蝇蛹集金小蜂寄生对寄主蛹可溶性蛋白与芳基蛋白组成与含量的影响

摘要/Abstract

摘要：

为了探讨蛹期寄生蜂对寄主蛋白代谢的寄生生理效应，利用Bradford蛋白含量测定法、Western免疫印迹法及酶联免疫吸附检测法研究了棕尾别麻蝇Boettcherisca peregrina蛹被丽蝇蛹集金小蜂Nasonia vitripennis寄生后其脂肪体和血淋巴中可溶性蛋白及芳基蛋白组成与含量的变化。结果表明：寄生蛹脂肪体和血淋巴中可溶性蛋白的组成与未寄生相比基本无明显差异；不论寄生与否寄主蛹脂肪体和血淋巴中芳基蛋白亚基分子量均为80 kDa，该亚基在脂肪体中未出现降解现象，而在血淋巴中仅于寄生后12 h的寄主蛹中呈现2条分子量相近的Western免疫印迹带，说明其降解可能先于未寄生对照。就含量而言，寄生蛹脂肪体中可溶性蛋白含量除寄生后24 h外均显著低于未寄生对照，芳基蛋白含量除寄生后48 h外也均显著低于未寄生对照，其中寄生后12 h的含量仅为未寄生的32.0%。寄生蛹血淋巴中可溶性蛋白含量多低于未寄生蛹，且寄生后2，12，24 h的差异达显著水平；芳基蛋白的含量均有低于未寄生的趋势，其中寄生后12 h的含量为未寄生的17.0%。综合认为，丽蝇蛹集金小蜂的寄生可导致寄主脂肪体和血淋巴中可溶性蛋白及芳基蛋白含量下降。

关键词: 丽蝇蛹集金小蜂, 棕尾别麻蝇, 可溶性蛋白, 芳基蛋白

Abstract: For further exploring the physiological effects of parastization by pupa-specific parasitoids on protein metabolic efficiency of hosts, the changes in compositions and contents of soluble proteins and arylphorin in the fat body and hemolymph of Boettcherisca peregrina pupae parasitized by Nasonia vitripennis were investigated by using the Bradford method for protein quantitation, Western blotting, and enzyme_linked immunosorbent assay. The results showed that the compositions of soluble proteins in the fat body and hemolymph of parasitized pupae were similar to those of non-parasitized pupae. There was a storage protein, namely arylphorin with a subunit molecular weight of 80 kDa, in the fat body and hemolymph of both parasitized and non-parasitized pupae. The arylphorin subunit was not degraded in the fat body of both parasitized and non-parasitized pupae, but was found to be broken up into

two bands with similar molecular weight only in the hemolymph of parasitized pupae at 12 h after parasitization by the Western blotting. This suggests that the arylphorin in the hemolymph might be broken down earlier in parasitized pupae than in non-parasitized pupae. The contents of soluble proteins in the fat body were significantly lower in parasitized pupae than in non-parasitized pupae at all the sampling stages except at 24 h after parasitization. In contrast, the contents of arylphorin in the fat body of parasitized pupae were also significantly lower than those of non-parasitized ones except at 48 h after parasitization, only 32.0% of that for nonparasitized pupae at 12 h after parasitization. The contents of soluble proteins in the hemolymph were more or less lower in parasitized pupae than in non-parasitized pupae, and their differences were significant at 2, 12 and 24 h after parasitization. There was a tendency that the content of arylphorin in the hemolymph was lower in parasitized pupae than in non-parasitized pupae, especially at 12 h after parasitization which was only 17% as high as that in non-parasitized pupae. In general, the results suggest that the parasitization by N. vitripennis may result in marked decreases in the contents of both soluble proteins and arylphorin in the fat body and hemolymph of hosts.

Key words: Nasonia vitripennis, Boettcherisca peregrina, soluble proteins, arylphorin, parasitization

韩成香，方琦，李凯，胡萃，叶恭银. 丽蝇蛹集金小蜂寄生对寄主蛹可溶性蛋白与芳基蛋白组成与含量的影响[J]. , 2008, 51(10): 1003-1010.

HAN Cheng-Xiang. Effects of parasitization by Nasonia vitripennis (Hymenoptera: Pteromalidae) on the compositions and contents of soluble proteins and arylphorin in host Boettcherisca peregrina (Diptera: Sarcophagidae) pupae[J]. , 2008, 51(10): 1003-1010.

[1]	王丽丽, 栾炳辉, 刘学卿, 王培松, 王英姿. 葡萄叶片中营养物质和叶绿素含量与其对绿盲蝽抗性的关系[J]. , 2017, 60(5): 570-575.
[2]	李培光, 邱仕祺, 叶保华, 王宁新, 黄大卫. 去除 Wolbachia 对丽蝇蛹集金小蜂繁殖适合度和成蜂寿命的影响[J]. , 2015, 58(9): 966-972.
[3]	钱岑，方琦，王磊，叶恭银. 丽蝇蛹集金小蜂Pacifastin蛋白酶抑制剂基因nvpp-1和nvpp-2的功能研究[J]. , 2013, 56(8): 841-853.
[4]	孙兴华, 周晓榕, 庞保平, 孟庆玖. 南美斑潜蝇为害对黄瓜体内主要营养物质、次生代谢物质及叶绿素含量的影响[J]. , 2012, 55(10): 1178-1184.
[5]	王林华, 黄翠, 黎路林. 一株棕尾别麻蝇胚胎细胞系的建立及其特性分析[J]. , 2011, 54(5): 515-521.
[6]	李艳敏，方琦，胡萃，叶恭银. 重金属Cd2+对棕尾别麻蝇血细胞数量、包囊作用和形态结构的影响[J]. , 2010, 53(9): 969-977.
[7]	吴国星，高熹，叶恭银，胡萃，程家安. 取食重金属铜对棕尾别麻蝇亲代及子代生长发育与繁殖的影响[J]. , 2007, 50(10): 1042-1048.
[8]	吴国星, 李凯, 叶恭银, 胡萃, 程家安. 棕尾别麻蝇金属硫蛋白的分离纯化及性质分析[J]. , 2006, 49(1): 22-28.
[9]	张忠，叶恭银，胡萃. 两种金小蜂毒液对菜粉蝶蛹血细胞延展、存活及包囊作用的影响[J]. , 2004, 47(5): 551-561.
[10]	张桂筠, 肖蔼祥. 蝇蛹俑小蜂、丽蝇蛹集金小蜂触角感器的扫描电镜观察[J]. , 1992, 35(2): 154-159.

丽蝇蛹集金小蜂寄生对寄主蛹可溶性蛋白与芳基蛋白组成与含量的影响

Effects of parasitization by Nasonia vitripennis (Hymenoptera: Pteromalidae) on the compositions and contents of soluble proteins and arylphorin in host Boettcherisca peregrina (Diptera: Sarcophagidae) pupae

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

Metrics

本文评价

推荐阅读 0