›› 2008, Vol. 51 ›› Issue (3): 298-306.

• 研究论文 • 上一篇    下一篇

基于Cytb和COⅠ基因部分序列的15种麻蝇之间的系统发育关系(双翅目:麻蝇科)

宋忠魁,王珣章,梁铬球   

  • 出版日期:2010-07-27 发布日期:2008-03-20
  • 通讯作者: 宋忠魁

Phylogenetic relationships among 15 sarcophagidfly species (Diptera: Sarcophagidae) based onpartial sequences of mitochondrial cytochromeb and cytochrome oxidase subunit Ⅰgenes

SONG Zhong-Kui   

  • Online:2010-07-27 Published:2008-03-20

摘要: 本研究通过测序Cytb基因和COⅠ基因的部分序列来推定15种麻蝇之间的系统发育关系。在世界麻蝇名录中,本研究的15种麻蝇能够代表麻蝇属Sarcophaga的6个亚属。连接序列(972 bp)被用于系统发育分析;分析方法包括了了最大简约法、最大似然法以及贝叶斯法。我们的结果提示了亚麻蝇亚属Parasarcophaga、别麻蝇亚属Boettcherisca以及红麻蝇亚属Liopygia的单系性,同时也表明蛇麻蝇亚属Liosarcophaga和德麻蝇亚属Pandelleisca并不是单源的。不过,目前的研究并不能分辨野德麻蝇S. (Pandelleisca) similis和峨眉叉麻蝇S. (Robineauella) coei的系统发育位置。此外,最大简约分析和似然功能分析在scopariiformis-iwuensis进化枝和polystylata-hui进化枝的关系上产生了不一致的系统发育推断。因此,后续研究不仅需要其他的分子标记,也需要更多的分类取样。

关键词: 贝叶斯分析, 系统发育, Cytb基因, COⅠ基因

Abstract: In this study, partial DNA sequences of two mitochondrial genes, cytochrome b (Cytb) and cytochrome c oxidase subunit (CO) genes, were  used to infer the phylogenetic relationships among 15 sarcophagid fly species, representing the six subgenera of the genus Sarcophaga in the catalogue of the Sarcophagidae of the world. The concatenated sequences (972 bp) were employed to carry out the phylogenetic analyses, including MP, ML, and Bayesian analyses. Our results suggested monophyly of three subgenera, i.e., the subgenus Parasarcophaga, the subgenus Boettcherisca, and the subgenus Liopygia. Our analyses also showed that the subgenus Liosarcophaga and the subgenus Pandelleisca were not monophyletic. This study showed no resolution for the phylogenetic positions of S. (Pandelleisca) similis and S. (Robineauella) coe. Furthermore, the MP analysis and the likelihood function analyses revealed incongruent phylogenetic relationship between the scopariiformisiwuensis clade and the polystylata hui clade. Therefore, other molecular markers and more taxon samplings were needed in the future study.

Key words: Bayesian analysis, phylogeny, cytochrome b (Cytb) gene, cytochrome oxidase subunitⅠ (COⅠ) gene