关键词: 直翅目, 蝗总科, 云斑车蝗, 线粒体基因组, RNA二级结构, 重复序列

Abstract: The complete sequence of Gastrimargus marmoratus (Thunberg) mitochondrial genome was determined using long PCR and conserved primers walking approaches. The results showed that the entire mitochondrial genome of G. marmoratus (GenBank accession no. EU527334) is 15 904 bp in length with 76.5% A+T content. It contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 tRNA genes, and an A+T-rich region of 1 057 bp. All protein-coding genes of the G. marmoratus mitochondrial genome start with a typical ATN codon, except for COⅠ and ND5 with TTG. The usual TAA and TAG termination codons were found for twelve protein-coding genes. However, the ND5 genes have an incomplete termination codon (T). With respect to the secondary structures of 22 tRNA genes, the absence of DHU arm of tRNA^Ser（AGN）was observed as in Locusta migratoria. tRNA^Ser（UGY）with 9 bases in anticodon loop was found, compared with typical 7 bases in others. The predicted secondary structures of 12S rRNA and 16S rRNA include 30 helices in 3 structural domains and 44 helices in 6 domains, respectively. The A+T content of the A+T-rich region is 76.5%, relatively higher than that of L. migratoria; three tandemly repeated DNA sequences were found in the A+T-rich region in G. marmoratus.

Key words: Orthoptera, Acridoidea, Gastrimargus marmoratus, mitochondrial genome, RNA secondary structure, tandemly repeated sequences