关键词: 转基因杨树, 几丁质酶基因, 蝎昆虫特异性神经毒素基因, 美国白蛾, 光肩星天牛, 抗虫性

Abstract: To verify the resistance of binary genes chitinase-BmkIT to larvae of Coleopterous insect Anoplophora glabripennis and Lepidopterous insect Hyphantria cunea, in vitro insect-resistant bioassays were conducted, where the 1^st-instar larvae of A. glabripennis were inoculated into poplar stems and those of H. cunea onto fresh poplar leaves. The results showed that transgenic clone 132-2 144-1 and 123-1 had significant lethal and inhibitory effects on H. cuneal larvae compared with the untransformed control （P＜0.05）. Most H. cunea larvae were not able to develop into pupae, some pupae were not able to emerge, and some emerged moths were abnormal with imperfect wings which died soon without mating. However, the differences in larval mortality and body weight of A. glabripennis were not significant compared with those of the untransformed control, indicating that transgenic stems had little influence on the survival and development of A. glabripennis larvae. The results suggested that the insect-resistant gene combination could not only be used as one of the complementary alien gene sources for resisting Lepidopterous pests but also contributed to reducing the risk of development of insect resistance produced by using single insect toxin gene.

Key words: Transgenic poplar, chitinase gene, BmkIT gene, Hyphantria cunea, Anoplophora glabripennis, insect resistance