›› 2008, Vol. 51 ›› Issue (8): 849-854.

• 研究论文 • 上一篇    下一篇



  • 出版日期:2010-07-27 发布日期:2008-08-20
  • 通讯作者: 黄大卫

Evolutionary analysis of CYP6 genes of Philotrypesis spp. (Hymenoptera: Chalcidoidea: Pteromalidae)

LIU Ying   

  • Online:2010-07-27 Published:2008-08-20

摘要: 采用PCR方法获得了佩妃延腹小蜂属Philotrypesis榕小蜂9CYP6基因片段:CYP6CK1, CYP6AQ2, CYP6AQ3, CYP6AS20, CYP6AS21, CYP6AS22, CYP6AS23, CYP6AS24 CYP6AS25这些基因序列都包含编码蛋白疏水螺旋区Ⅰ和血红素结合域的区域。使用MEGA 4.0构建基因树,用PAML 4.0检测选择压力。结果表明:榕小蜂的CYP6AS, CYP6AQ, CYP6CK基因亚家族的扩张可能是在自然选择下通过基因复制方式完成的。基于最大似然法的位点特异性模型对蛋白编码序列选择压力检测发现:榕小蜂CYP6AS亚家族的基因接受净化选择,在氨基酸序列上表现出较高的保守性。

关键词: 佩妃延腹小蜂属, 细胞色素P450, CYP6基因家族, 基因复制, 净化选择

Abstract: Nine new DNA fragments encoding cytochrome P450 were amplified from the fig wasps Philotrypesis spp.:  CYP6CK1, CYP6AQ2, CYP6AQ3, CYP6AS20, CYP6AS21, CYP6AS22, CYP6AS23, CYP6AS24 and CYP6AS25. These sequences include helix and heme-binding regions which are important functional regions for all P450 genes. We employed MEGA 4.0 to construct gene tree and PAML 4.0 to test the selection pressure on the encoding region. The results showed that the expansion of CYP6AS, CYP6AQ and CYP6CK subfamilies might be produced by the events of gene duplication. Members in the CYP6AS subfamily from those fig wasps are suffering from purifying selection, and the encoding regions are highly conservative.

Key words: Philotrypesis, cytochrome P450, CYP6 family, gene duplication, purifying selection