F1代法监测田间棉铃虫对转Bt基因棉的抗性

›› 2008, Vol. 51 ›› Issue (9): 938-945.

F1代法监测田间棉铃虫对转Bt基因棉的抗性

刘凤沂，朱玉成，沈晋良

出版日期:2008-09-20 发布日期:2008-09-20
通讯作者: 沈晋良

Monitoring resistance to transgenic Bt cotton in field populations of Helicoverpa armigera (Hübner) (Lepidoptera：Noctuidae) with F1 screening method

LIU Feng-Yi

Online:2008-09-20 Published:2008-09-20

摘要/Abstract

摘要： 采用表达单价Cry1Ac毒素的转Bt基因棉棉叶喂饲法比较了棉铃虫Helicoverpa armigera(Hübner)抗、感亲本及正反交后代在Bt棉上5 d的发育速率，确定棉铃虫在Bt棉上5 d发育到2龄中期以上、体重≥0.6 mg的个体作为判别抗性纯合子的标准。2006年采用F₁代法在室内用Bt棉叶喂饲法检测河北省邱县Bt棉田棉铃虫对Bt棉的抗性等位基因频率。结果表明，127头田间雄虫中24头携带抗性基因，估测抗性等位基因频率为0.94（95%CI：0.044～0.145），该值为在国内首次检测到的高抗性等位基因频率。该地区长期大面积种植转单价Bt棉和缺少非Bt棉作为有效庇护区导致田间抗性快速进化，需要尽快采取有效的抗性治理策略。本文还讨论了影响大田产生抗性的因素以及田间存在的抗性风险等。

关键词: 转Bt基因棉, 棉铃虫, 性监测, F1代法

Abstract: Development of Bt-susceptible and resistant Helicoverpa armigera and their reciprocal crosses feeding on Bt cotton leaves for 5 d were compared，and individuals ≥0.6 mg/larva and surviving beyond the mid-2nd-instar on Bt cotton leaves for 5 d were considered as the standard for homozygous resistant individuals. In 2006，the frequency of allele conferring resistance to Bt cotton in Qiuxian County，Hebei Province，was estimated by using the F₁ screening method. The results showed that 24 out of 127 field-collected male moths were detected to carry resistance alleles and the resistance allele frequency was estimated as 0.094 （95%CI： 0.044–0.154）. This is the first report that resistance allele frequency increases to such a high level in fields in China. Long-term adoption of Bt sprays，dominant planting of uni-toxin-producing Bt cotton，and lack of conventional cotton refuge system may accelerate the resistance evolution in this region，and it is necessary to establish and implement effective resistance management strategy as soon as possible. Factors influenced resistance evolution and the resistance risk in fields were discussed as well.

Key words: Cotton bollworm (Helicoverpa armigera), transgenic Bt cotton, resistance monitoring, F1 screening method

刘凤沂，朱玉成，沈晋良. F1代法监测田间棉铃虫对转Bt基因棉的抗性[J]. , 2008, 51(9): 938-945.

LIU Feng-Yi. Monitoring resistance to transgenic Bt cotton in field populations of Helicoverpa armigera (Hübner) (Lepidoptera：Noctuidae) with F1 screening method[J]. , 2008, 51(9): 938-945.

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