›› 2008, Vol. 51 ›› Issue (9): 986-991.

• 研究论文 • 上一篇    下一篇

β-细辛醚对谷蠹成虫体内四种酶活性的影响

宋旭红,邱艳,黄衍章,华红霞,蔡万伦,杨长举,薛东   

  • 出版日期:2008-09-20 发布日期:2008-09-20
  • 通讯作者: 华红霞

Effect of β-asarone on the activities of four enzymes in adults of Rhizopertha dominica (Fabricius) (Coleoptera: Bostrichidae)

SONG Xu-Hong   

  • Online:2008-09-20 Published:2008-09-20

摘要: 采用石菖蒲Acorus gramineus根茎提取物β-细辛醚对谷蠹Rhizopertha dominica成虫进行拌粮处理,测定β-细辛醚对谷蠹体内乙酰胆碱酯酶、谷胱甘肽S-转移酶、羧酸酯酶和酯酶同工酶的时间效应和剂量效应。结果表明:β-细辛醚对试虫体内4种酶的酶活性均表现出较强的时间效应。LC50(94.49 mg/kg)剂量的β-细辛醚处理后,谷蠹成虫体内乙酰胆碱酯酶和谷胱甘肽S-转移酶活性随处理时间的延长整体表现为抑制作用,对羧酸酯酶和酯酶同工酶则表现诱导增加作用。低剂量(67.5 mg/kg)β-细辛醚对乙酰胆碱酯酶具有显著的诱导作用,但是随着处理剂量的升高,对乙酰胆碱酯酶的活力多数表现为抑制作用。低剂量(≤100.0 mg/kg)β-细辛醚对谷胱甘肽S转移酶具有诱导作用,而高剂量(≥133.3 mg/kg)β-细辛醚对谷胱甘肽S-转移酶具有抑制作用。β-细辛醚对羧酸酯酶的活性多数表现为诱导作用,提高β-细辛醚的处理剂量可提高羧酸酯酶的活力。不同剂量的β-细辛醚处理对谷蠹酯酶同工酶均具有显著的诱导作用,但诱导效果与处理剂量关系并不显著。

关键词: 谷蠹, 石菖蒲, β-细辛醚, 乙酰胆碱酯酶, 谷胱甘肽S-转移酶, 羧酸酯酶, 酯酶同工酶

Abstract: Four important enzymes including acetylcholinesterase (AChE), glutathione-S-transferase (GSTs), carboxylesterase (CarE) and esterase isoenzyme (EST isoenzyme) in Rhizopertha dominica adults were studied after treated with β-asarone extracted from Acorus gramineus roots and shoots. The results indicated that activities of the four enzymes showed strong time-effect. Activities of AChE and GSTs were inhibited by β-asarone at a dosage of 94.49 mg/kg with the exposure time prolonged. While the activities of CarE and EST isoenzymes were induced by β-asarone at the same dosage. Theβ-asarone at a low dosage (67.5 mg/kg) showed marked induction effect on AChE activity. However, with the dosages of β-asarone increasing, AChE activity was mostly restrained. β-asarone showed induction effect on GST at a low dosage (100.0 mg/kg), but showed inhibitory effect at a high dosage (133.3 mg/kg). With the dosages of β-asarone increasing, the CarE activity was somewhat promoted.β-asarone induced EST isozyme activity at different dosages, but the induction effect was not significantly correlated with the treated dosages.

Key words: Rhizopertha dominica, Acorus gramineus, β-asarone, acetylcholinesterase, glutathione-S-transferase, carboxylesterase, esterase isoenzyme