›› 2009, Vol. 52 ›› Issue (2): 121-125.

• 研究论文 •    下一篇



  • 出版日期:2009-03-18 发布日期:2009-02-20
  • 通讯作者: 曲明静

EAG and behavioral responses of the large black chafer, Holotrichia parallela (Coleoptera: Scarabaeidae) to its sex pheromone

  • Online:2009-03-18 Published:2009-02-20

摘要: 为了分离鉴定暗黑鳃金龟的性信息素成分并对其功能进行验证,本研究对比摸索了3种触角的处理方法,并进行同一浓度(30 ng·μL-1)二元混合物、不同配比的触角EAG测试。结果包括: 建立了一种鳃叶状触角的触角电位(EAG)测定方法,即将暗黑鳃金龟Holotrichia parallela触角的各鳃叶用针分离开后进行测定,这种方法测得的触角电位反应值较高。雄虫触角对L-异亮氨酸甲酯和(R)-(-)-芳樟醇为7∶1的二元混合物的反应值最高,和暗黑鳃金龟雌虫性信息素25个腺体提取液的EAG反应相当;对同一配比(7∶1)不同剂量刺激液的EAG测试表明,雄虫对浓度为30 ng·μL-1的二元混合物刺激液的反应值最高。嗅觉反应结果进一步证实,试虫对L-异亮氨酸甲酯和(R)-(-)-芳樟醇7∶1的选择最高,选择系数达93.3%。研究结果为利用性信息素防治暗黑鳃金龟技术的开发提供了基础。

关键词: 暗黑鳃金龟, 性信息素, 触角电位, 嗅觉反应, L-异亮氨酸甲酯, (R)-(-)-芳樟醇

Abstract: A method measuring EAG (electroantennogram) response in lamellate antennae of Holotrichia parallela Motschulsky was established in this study. A higher value of EAG was got when lamellae were separated from each other by insect pin. With the concentration of 30 ng·μL-1, the EAG value of male antennae reached the maximum when the volume ratio of L-isoleucine methyl ester (LIME) to (R)-(-)-linalool was 7∶1, which was almost equal to the EAG value of the crude extracts from 25 sex glands. The male antennae were treated with different concentrations of stimulative reagent with the fixed volume ratio (7∶1) of LIME to (R)-(-)-linalool. The highest EAG value of male antennae was found when the concentration was 30 ng·μL-1. The results of olfactory response further confirmed that male H. parallela was attracted with the selection efficiency of 93.3% when the volume ratio of  LIME  to (R)-(-)-linalool was 7∶1. So it can be deduced that the sex pheromone of Holotrichia parallela in Shandong province were the mixture of  LIME  and (R)-(-)-linalool with the ratio of 7∶1. The results may serve as basis for developing the techniques of sex pheromone attraction to control H. parallela.

Key words: Holotrichia parallela, sex pheromone, EAG, olfactory response, L-isoleucine methyl ester