›› 2009, Vol. 52 ›› Issue (2): 133-139.

• 研究论文 • 上一篇    下一篇

麦红吸浆虫不同滞育期四种糖代谢酶活力分析

成卫宁,李修炼,李怡萍,李建军,仵均祥   

  • 出版日期:2009-03-18 发布日期:2009-02-20
  • 通讯作者: 仵均祥

Activities of four sugar metabolic enzymes in Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) larvae at different diapause stages

  • Online:2009-03-18 Published:2009-02-20

摘要: 海藻糖是麦红吸浆虫Sitodiplosis mosellana (Gehin)滞育期间储藏能量的主要物质,为弄清其在滞育期的积累机理,本文测定了麦红吸浆虫滞育前后和滞育期糖原磷酸化酶(Gpase)、己糖激酶(HK)、磷酸果糖激酶(PFK)和醛缩酶(ALD)这4种糖代谢酶活力的变化。结果表明: 麦红吸浆虫滞育前后这些糖代谢酶活力明显不同,滞育后Gpase 活力显著提高,糖酵解有关酶HK,PFK和ALD活力降低;滞育解除后,Gpase 活力降低,HK,PFK和ALD活力升高。滞育期间,4种糖代谢酶的活力均与滞育发育有关;同时Gpase 和PFK的活力也与环境温度有关,即夏、冬季高于春、秋季;同期不同滞育状态幼虫比较,裸露幼虫HK,PFK和ALD活力总是略高于结茧幼虫,Gpase则相反。滞育当年与第2年同期幼虫4种糖代谢酶的活力无显著差异。

关键词: 麦红吸浆虫, 滞育, 海藻糖, 糖代谢酶, 酶活力

Abstract:

In order to reveal the accumulation mechanism of trehalose, a main chemical substance to maintain diapause of Sitodiplosis mosellana (Gehin), we measured the activities of four sugar metabolic enzymes including glycogen phosphorylase (Gpase), hexokinase (HK), phosphofructokinase (PFK) and aldolase (ALD) in pre-diapause, diapause and post-diapause larvae of S. mosellana by using testing kits. The results indicated that activities of these sugar metabolic enzymes were obviously different at various diapause stages of S. mosellana larvae. Gpase activity was increased significantly, while activities of glycolysis enzymes (HK, ALK and PFK) were decreased significantly after entering diapause. Gpase activity was decreased, while activities of glycolysis enzymes were increased after diapause termination. Activities of four enzymes were all related with developmental status of diapause larvae during diapause. Gpase and PFK were also influenced by environmental temperature, namely, activities of Gpase and PFK were higher in summer and winter than those in spring and autumn. At the same diapause stage, activities of HK, PFK and ALK of non-cocooned larvae were higher than those of cocooned larvae. On the contrary, Gpase activity of non-cocooned larvae was lower than that of cocooned larvae. No obvious difference in four metabolic enzyme activities was found between the 1st and 2nd year diapause larvae.

Key words: Sitodiplosis mosellana, diapause, trehalose, sugar metabolic enzymes, enzyme activity