›› 2009, Vol. 52 ›› Issue (2): 191-201.

• 研究论文 • 上一篇    下一篇

基于线粒体ND1和16S rRNA基因序列探讨中国蝴蝶12科的系统发育关系(鳞翅目: 双孔次亚目: 蝶类)

邹方振, 郝家胜, 黄敦元, 张大秀, 朱国萍, 朱朝东   

  • 出版日期:2009-03-18 发布日期:2009-02-20
  • 通讯作者: 郝家胜,朱朝东

Molecular phylogeny of 12 families of the Chinese butterflies based on mitochondrial ND1 and 16S rRNA gene sequences (Lepidoptera: Ditrysia: Rhopalocera)

  • Online:2009-03-18 Published:2009-02-20

摘要: 对中国12科共32种代表蝶类的ND1基因和16S rRNA 基因进行了序列测定(包括新测30种ND1基因和9种16S rRNA基因)和比较分析, 同时采用邻接法、最大似然法和贝叶斯法构建了12科蝶类的系统发育树, 探讨了其高级分类群的系统发育关系。序列分析的结果显示: 经比对处理后的两个基因总长度为869 bp, 其中保守位点373个, 可变位点496个, 简约信息位点375个; A+T的平均含量为80.2%, 明显高于C+G的平均含量19.8%。分子系统树表明: 蛱蝶科不是单系群; 珍蝶类、斑蝶类和喙蝶类位于蛱蝶科内; 粉蝶科和凤蝶科具有共同祖先。据此建议: 绢蝶科应归入凤蝶科; 蚬蝶科归入灰蝶科; 珍蝶类、斑蝶类和喙蝶类作为蛱蝶科中的亚科, 眼蝶类从蛱蝶科中分离出来独立成科。另外, 环蝶类的系统分类地位还有待于进一步研究。

关键词: 鳞翅目, 双孔次亚目, 蝶类, ND1基因, 16S rRNA 基因, 系统发育

Abstract: In this study, the phylogenetic relationships of 12 families of the Chinese butterflies were investigated based on mitochondrial ND1 and 16S rRNA gene sequences. Thirty-two Chinese species were included in the analyses, of which 30 species were sequenced for the ND1 and 9 for the 16S rRNA gene. In the alignment, the two combined genes were 869 bp in length, including 373 conserved, 496 variable and 375 parsimonious-informative sites, respectively. The average percentage of A+T is 80.2%, which is much higher than that of C+G (19.8%). With both Zygaena angelicae and Z. transalpina as the outgroup taxa, the phylogenetic trees were reconstructed with the neighbor-joining (NJ), maximum-likelihood (ML) and Bayesian inference (BI) methods. Major results included that: 1) Nymphalidae is not monophyletic; 2) the acraeids, danaids and libytheids are included in Nymphalidae; 3) the Pieridae and Papilionidae have a closest common ancestor; 4) the Parnassiidae should be included in Papilionidae; and 5) the satyrids should be separated from the Nymphalidae and be given a family rank. In addition, our results also suggest that the Riodinidae should be classified into Lycaenidae, and the acraeids should be given a subfamily rank. As to the amathusiidae, its taxonomic level and phylogentic position need further more detailed studies.

Key words: Lepidoptera, Ditrysia, Rhopalocera, mitochondrial ND1 gene, 16S rRNA gene, phylogeny