12a-羟基鱼藤酮对斜纹夜蛾生殖力的影响及其作用机理

摘要/Abstract

摘要： 为探讨12a-羟基鱼藤酮对斜纹夜蛾Prodenia litura (Fabricius)生殖力的影响及其作用机理, 本文测定了该虫取食12a-羟基鱼藤酮后的产卵量。在用高效液相色谱仪确定其卵巢组织中存在12a-羟基鱼藤酮后, 通过MTT法测定该化合物对斜纹夜蛾卵巢细胞（PL细胞）的细胞毒性，并利用流式细胞仪检测该化合物对PL细胞细胞周期、膜电位、胞内钙离子浓度、线粒体膜电位的影响。结果表明:斜纹夜蛾幼虫取食12a-羟基鱼藤酮后产卵量下降80％以上, 并从卵巢组织中检测出该化合物。12a-羟基鱼藤酮对PL细胞增殖具有抑制活性, 其IC₅₀为6.6 mg/L。该化合物将PL细胞周期阻滞于S期, 导致PL细胞膜电位、线粒体膜电位和细胞内游离钙离子浓度均显著升高。该化合物导致斜纹夜蛾繁殖力降低。12a-羟基鱼藤酮将PL细胞增殖阻滞于细胞周期的S期。同时, 该化合物对卵巢细胞具有较弱的毒杀活性, 导致一些卵巢细胞死亡。由于上述原因, 卵巢细胞数量逐渐减少而导致卵巢管萎缩。卵巢管的萎缩使卵巢管内的卵母细胞不能发育成卵细胞, 故其生殖力下降。

关键词: 斜纹夜蛾, 12a-羟基鱼藤酮, 繁殖力, 细胞毒性, 细胞周期, 钙离子浓度, 线粒体膜电位

Abstract: In order to study the effect of 12a-hydroxyrotenone on the fecundity of Prodenia litura (Fabricius) and its action mechanisms, the number of eggs laid per female adult of P. litura was investigated; after 12a-hhydroxyrotenone in ovary was determined with HLPC, its cytotoxicity to ovarian cells from P. litura(PL cells) was determined at 48 h after treatment by MTT assay. The effect of 12a-hydroxyrotenone on cell cycle, cell membrane potential and mitochondrial membrane potential of PL cells, and intracellular free calcium concentration in PL cells at 24 h after treatment was also tested by flow cytometry. The results showed that the number of eggs laid per female adult declined by above 80% after 12a-hydroxyrotenone was fed to P. litura larvae, and the compound could be detected from the ovarian tissues. 12a-hydroxyrotenone showed inhibitory activity to PL cells, with IC₅₀being 6.6 mg/L at 48 h after exposure. After PL cells were treated with 12a-hydroxyrotenone for 24 h, the percentage of S phase cells markedly increased in cultured PL cells (P<0.05), while the percentage of G₂-M phase cells decreased. Cell membrane potential, mitochondrial membrane potential and the intracellular free calcium concentration in PL cells were significantly higher than those of the control groups. 12a-hydroxyrotenone led to a linear decrease in the number of eggs laid per female and PL cell cycle arrest in S phase. 12a-hydroxyrotenone exhibited weak cytotoxicity to PL cells, and caused some of them to death. Thus the number of ovarian cells decreased, and the ovary development was inhibited so that ovarian cells could not develop into mature ooctyes and its fecundity obviously decreased.

Key words: Prodenia litura, 12a-hydroxyrotenone, fecundity, cytotoxicity, cell cycle, intracellular free calcium concentration, mitochondrial membrane potential

李有志, 杨海君, 徐汉虹, 刘仲华, 高必达, 张瑞峰, 周志成. 12a-羟基鱼藤酮对斜纹夜蛾生殖力的影响及其作用机理[J]. , 2009, 52(3): 267-273.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/

http://www.insect.org.cn/CN/Y2009/V52/I3/267

[1]	岳文波, 郅军锐, 刘利, 叶茂, 张向前, 曾广 . 害虫取食和机械损伤对菜豆不同部位叶片防御酶活性的影响[J]. , 2018, 61(7): 860-870.
[2]	孙佳斌, 刘乃勇, 李双美, 闫祺, 董双林. 斜纹夜蛾信息素结合蛋白SlitPBP4的分子克隆、组织表达谱及结合特性分析[J]. , 2018, 61(6): 657-667.
[3]	武德功, 詹秋文, 黄保宏, 王增霞, 黄伟东, 毕亚玲, 刘长仲, 杜军利. 不同光周期对高粱蚜种群参数的影响[J]. , 2018, 61(4): 511-518.
[4]	任小双, 张兰, 张燕宁, 毛连纲, 蒋红云. 喜树碱诱导甜菜夜蛾细胞凋亡线粒体途径的MPTP依赖性[J]. , 2017, 60(10): 1105-1113.
[5]	梁炜博, 向兴, 王学贵, 杨群芳, 罗家栋. 噻虫啉亚致死剂量对斜纹夜蛾解毒酶系活性与生长繁殖的影响[J]. , 2017, 60(10): 1187-1197.
[6]	庾琴, 王芳, 张润祥, 郭贵明, 范仁俊, 郝赤. 梨小食心虫蛹重对成虫繁殖力和寿命及下一代幼虫发育的影响[J]. , 2016, 59(9): 985-990.
[7]	范琳芳, 何文婧, 苏智仁, 朱家明, 罗威, 张纯, 黄立华. 斜纹夜蛾酵母双杂交文库的构建及其在蜕皮激素受体互作蛋白筛选中的应用[J]. , 2016, 59(8): 801-811.
[8]	郭成林, 覃柳燕, 万树青, 马跃峰, 覃建林, 马永林. 黄皮种子提取物对斜纹夜蛾幼虫的杀虫活性及有效成分鉴定[J]. , 2016, 59(8): 839-845.
[9]	郝强, 黄倩, 梁炜博, 贡常委, 王学贵. 不同温度下斜纹夜蛾的两性生命表[J]. , 2016, 59(6): 654-662.
[10]	李娜, 范凡, 韩慧, 裴晓亚, 刘顺, 魏国树. 不同波长LED光源对韭菜迟眼蕈蚊生殖行为的影响[J]. , 2016, 59(5): 546-551.
[11]	金桂华, 宫亚军, 潜宗伟, 朱亮, 王泽华, 陈金翠, 魏书军. 二斑叶螨对不同品种茄子的选择性与适合度[J]. , 2016, 59(3): 328-336.
[12]	刘佳, 周勇, 朱航, 马海昊, 邓希乐, 周小毛, 柏连阳. 斜纹夜蛾抗药性监测及茚虫威对其解毒代谢酶的影响[J]. , 2016, 59(11): 1254-1262.
[13]	李志文, 孙寒松. 湖南益阳小果油茶林小果油茶、油茶象和啮齿动物的相互作用[J]. , 2016, 59(10): 1123-1132.
[14]	左太强, 张彬, 张绍婷, 郑长英, 万方浩. 高温和啶虫脒处理西花蓟马对其F₁代生命表参数的联合作用[J]. , 2015, 58(7): 767-775.
[15]	唐维媛, 邢丛丛, 董永刚, 王啸, 张义明. 斜纹夜蛾幼虫感染莱氏野村菌后的抗氧化酶活性变化[J]. , 2015, 58(5): 526-534.

12a-羟基鱼藤酮对斜纹夜蛾生殖力的影响及其作用机理

Effect of 12a-hydroxyrotenone on the fecundity of Prodenia litura (Fabricius) (Lepidoptera: Noctuidae) and its mechanisms

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0